[1]盛世美,张建刚,陈烨,等.金线莲多糖通过抑制NF-κB信号通路对糖尿病小鼠肾损伤的改善作用[J].华侨大学学报(自然科学版),2024,45(5):635-641.[doi:10.11830/ISSN.1000-5013.202402003]
　SHENG Shimei,ZHANG Jiangang,CHEN Ye,et al.Improvement Effects of Anoectochilus roxburghii Polysaccharose on Renal Injury in Diabetic Mice by Inhibiting NF-кB Signaling Pathway[J].Journal of Huaqiao University(Natural Science),2024,45(5):635-641.[doi:10.11830/ISSN.1000-5013.202402003]
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金线莲多糖通过抑制NF-κB信号通路对糖尿病小鼠肾损伤的改善作用()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第45卷
期数:
2024年第5期
页码:
635-641
栏目:
出版日期:
2024-09-20

文章信息/Info

Title:
Improvement Effects of Anoectochilus roxburghii Polysaccharose on Renal Injury in Diabetic Mice by Inhibiting NF-кB Signaling Pathway
文章编号:
1000-5013(2024)05-0635-07
作者:
盛世美1;  张建刚2;  陈烨1;  戴招龙1;  李乐1;  刘青1
1. 华侨大学 化工学院, 福建 厦门 361021;2. 兰州大学 基础医学院, 甘肃 兰州 730000
Author(s):
SHENG Shimei1;  ZHANG Jiangang2;  CHEN Ye1; DAI Zhaolong1;  LI Le1;  LIU Qing1
1. College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2. School of Basic Medicine, Lanzhou University, Lanzhou 73/000, China
关键词:
金线莲多糖;  糖尿病;  肾损伤;  NF-κB信号通路
Keywords:
Anoectochilus roxburghii polysaccharose;  diabetes;  renal injure;  NF-κB signaling pathway
分类号:
R965.1
DOI:
10.11830/ISSN.1000-5013.202402003
文献标志码:
A
摘要:
为了考察金线莲多糖对糖尿病小鼠肾损伤的改善作用,将糖尿病小鼠随机分为模型组、二甲双胍组及高、低剂量金线莲多糖组(100和300 mg·kg-1),给药4周后测定小鼠血清生化指标、炎症因子及肾皮质中的蛋白表达,并观察肾组织形态学变化。结果表明:与正常组比,模型组小鼠24 h的尿蛋白质量,血肌酐浓度、丙二醛浓度、炎症因子显著升高,而血清超氧化物歧化酶活力显著下降;模型组小鼠肾组织p-NF-κB p65的蛋白表达显著上调,podocin,Nephrin的蛋白表达明显下调;组织学检查显示模型组小鼠肾组织结构异常改变;经金线莲多糖干预后,糖尿病小鼠血清生化指标、肾组织中蛋白表达均明显逆转,组织学检查也显示糖尿病小鼠肾损伤明显改善。
Abstract:
To investigate the improvement effect of Anoectochilus roxburghii polysaccharide on renal injury in diabetic mice, the diabetic mice were randomly divided into model group, metformin group and Anoectochilus roxburghii polysaccharide groups(100 and 300 mg·kg-1). After 4 weeks of treatment, the serum biochemical indexes, inflammatory factors and protein expression in renal cortex of the mice were measured, and the renal morphological changes were observed. The results showed that compared with the control group, 24-hour urine protein mass, serum creatinine concentration, malondialdehyde content and inflammatory factors of mice in model group were significantly increased, while serum superoxide dismutase activity was significantly decreased. The protein expression of p-NF-κB p65 in renal tissue of model group was significantly up-regulated, and the protein expressions of podocin and Nephrin were significantly down-regulated. Histological examination showed abnormal changes of renal tissue structure of mice in model group. The serum biochemical indexes and protein expression in kidney tissue of diabetic mice were significantly reversed after the intervention of the Anoectochilus roxburghii polysaccharide, and the histological examination also showed that the kidney injury of diabetic mice treated with Anoectochilus roxburghii polysaccharide was significantly improved.

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备注/Memo

备注/Memo:
收稿日期: 2024-02-15
通信作者: 刘青(1970-),女,副教授,博士,主要从事药理及中药提取物的活性研究。E-mail:liuq@hqu.edu.cn。https://hdxb.hqu.edu.cn/
更新日期/Last Update: 2024-09-20