[1]吴冰,段又愈,陈爱政.超临界CO2流体技术制备脱细胞外基质材料及其生物医学应用[J].华侨大学学报(自然科学版),2024,45(5):551-558.[doi:10.11830/ISSN.1000-5013.202407001]
　WU Bing,DUAN Youyu,CHEN Aizheng.Supercritical CO2 Fluid Technology-Assisted Fabrication of Decellularized Extracellular Matrix Materials and Biomedical Applications[J].Journal of Huaqiao University(Natural Science),2024,45(5):551-558.[doi:10.11830/ISSN.1000-5013.202407001]
点击复制

超临界CO2流体技术制备脱细胞外基质材料及其生物医学应用()
分享到：

《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第45卷
期数:
2024年第5期
页码:
551-558
栏目:
出版日期:
2024-09-20

文章信息/Info

Title:
Supercritical CO2 Fluid Technology-Assisted Fabrication of Decellularized Extracellular Matrix Materials and Biomedical Applications
文章编号:
1000-5013(2024)05-0551-08
作者:
吴冰;  段又愈;  陈爱政
华侨大学 化工学院, 福建 厦门 361021
Author(s):
WU Bing;  DUAN Youyu;  CHEN Aizheng
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
超临界CO2流体;  脱细胞外基质;  超临界灭菌;  组织工程
Keywords:
supercritical carbon dioxidefluid;  decellularized extracellular matrix;  supercritical sterilization;  tissue engineering
分类号:
R318
DOI:
10.11830/ISSN.1000-5013.202407001
文献标志码:
A
摘要:
脱细胞外基质(decellularized extracellular matrix,dECM)材料在组织工程和再生医学中具有重要应用,超临界CO2流体(supercritical CO2 fluid, SCF-CO2)技术已被证明可用于dECM材料的制备。文中综述了SCF-CO2的基本原理、SCF-CO2脱细胞及其灭菌机制,介绍了辅助溶剂的使用与预处理技术对SCF-CO2脱细胞的影响,以及dECM材料的生物医学应用。结果表明:与常规方法相比,具有高渗透性、高扩散性的SCF-CO2能更有效地保持细胞外基质(extracellular matrix,ECM)的结构完整性和生物活性,同时展现出高效灭菌的优势,且制备的dECM材料在组织修复和再生医学领域具有显著应用潜力。
Abstract:
Decellularized extracellular matrix(dECM)materials play a significant role in tissue engineering and regenerative medicine. Supercritical CO2 fluid(SCF-CO2)technology has been proven to be applicable in the preparation of dECM materials. By reviewing relevant literature in recent years, this paper summarizes the basic principles of SCF-CO2, the mechanisms of decellularization and sterilization by SCF-CO2, and discusses the impact of auxiliary solvents and pretreatment techniques on SCF-CO2-assisted decellularization, as well as the biomedical applications of dECM materials. The results show that, compared with conventional methods, SCF-CO2 with high permeability and high diffusivity could maintain the structural integrity and biological activity of extracellular matrix(ECM)more effectively and show the advantage of high sterilization efficiency. Moreover, the prepared dECM materials have significant potential for application in the field of tissue repair and regenerative medicine.

参考文献/References:

[1] SEO Y,JUNG Y,KIM S H.Decellularized heart ECM hydrogel using supercritical carbon dioxide for improved angiogenesis[J].Acta Biomaterialia,2018,67:270-281.DOI:10.1016/j.actbio.2017.11.046.
[2] COUNSELL J F,EVERETT D H.Nature of fluids in the hypercritical region[J].Nature,1960,188(4750):576-577.DOI:10.1038/188576a0.
[3] YOUSEFI M,RAHIMI NASRABADI M,POURMORTAZAVI S M,et al.Supercritical fluid extraction of essential oils[J].Trends in Analytical Chemistry,2019,118:182-193.DOI:10.1016/j.trac.2019.05.038.
[4] 吕东灿,刘运权,王夺,等.生物油萃取分离技术的研究进展[J].化工进展,2012,31(7):1425-1431.DOI:10.16085/j.issn.1000-6613.2012.07.025.
[5] FRANCO P,DE MARCO I.Nanoparticles and nanocrystals by supercritical CO2-assisted techniques for pharmaceutical applications:areview[J].Applied Sciences,2021,11(4):1476.DOI:10.3390/app11041476.
[6] DONG Mengyao,WANG Gang,ZHANG Xiangning,et al.An overview of polymer foaming assisted by supercritical fluid[J].Advanced Composites and Hybrid Materials,2023,6(6):207.DOI:10.1007/s42114-023-00790-6.
[7] KANKALA R K,ZHANG Y S,WANG Shibin,et al.Supercritical fluid technology: An emphasis on drug delivery and related biomedical applications[J].Advanced Healthcare Materials,2017,6(16):1700433.DOI:10.1002/adhm.201700433.
[8] DE WIT R J J,VAN DIS D J,BERTRAND M E,et al.Scaffold-based tissue engineering: Supercritical carbon dioxide as an alternative method for decellularization and sterilization of dense materials[J].Acta Biomaterialia,2023,155:323-332.DOI:10.1016/j.actbio.2022.11.028.
[9] BEJLERI D,DAVIS M E.Decellularized extracellular matrix materials for cardiac repair and regeneration[J].Advanced Healthcare Materials,2019,8(5):1801217.DOI:10.1002/adhm.201801217.
[10] YAO Qing,ZHENG Yawen,LAN Qinghua,et al.Recent development and biomedical applications of decellularized extracellular matrix biomaterials[J].Materials Science and Engineering: C,2019,104:109942.DOI:10.1016/j.msec.2019.109942.
[11] KIM B S,KIM J U,LEE JW,et al.Comparative analysis of supercritical fluid-based and chemical-based decellularization techniques for nerve tissue regeneration[J].Biomaterials Science,2024,12(7):1847-1863.DOI:10.1039/D3BM02072J.
[12] KANG Xing,MAO Liuhao,SHI Jinwen,et al.Supercritical carbon dioxide systems for sustainable and efficient dissolution of solutes:a review[J].Environmental Chemistry Letters,2024,22(2):815-839.DOI:10.1007/s10311-023-01681-4.
[13] CHASCHIN I S,BRITIKOV D V,KHUGAEV G A,et al.Decellularization of the human donor aortic conduit by a new hybrid treatment in a multicomponent system with supercritical CO2 and Tween 80[J].The Journal of Supercritical Fluids,2022,180:105452.DOI:10.1016/j.supflu.2021.105452.
[14] SAWADA K,TERADA D,YAMAOKA T,et al.Cell removal with supercritical carbon dioxide for acellular artificial tissue[J].Journal of Chemical Technology & Biotechnology,2008,83(6):943-949.DOI:10.1002/jctb.1899.
[15] CHEN Yiting,LEE H S,HSIEH D J,et al.3D composite engineered using supercritical CO2 decellularized porcine cartilage scaffold,chondrocytes,and PRP:Role in articular cartilage regeneration[J].Journal of Tissue Engineering and Regenerative Medicine,2021,15(2):163-175.DOI:10.1002/term.3162.
[16] KIM B S,KIM J U,LEE J W,et al.Comparative analysis of supercritical fluid-based and chemical-based decellularization techniques for nerve tissue regeneration[J].Biomaterials Science,2024,12(7):1847-1863.DOI:10.1039/D3BM02072J.
[17] ANTONS J,MARASCIO M G M,AEBERHARD P,et al.Decellularised tissues obtained by a CO2-philic detergent and supercritical CO2[J].European Cells and Materials,2018,36:81-95.DOI:10.22203/eCM.v036a07.
[18] TOPUZ B,GüNAL G,GULER S,et al.Chapter 4 - Use of supercritical CO2 in soft tissue decellularization [M]//CABALLERO D,KUNDU S C,REIS R L.Methods in Cell Biology.Academic Press.2020:49-79.DOI:10.1016/bs.mcb.2019.10.012.
[19] GULER S,ASLAN B,HOSSEINIAN P,et al.Supercritical carbon dioxide-assisted decellularization of aorta and cornea[J].Tissue Engineering Part C: Methods,2017,23(9):540-547.DOI:10.1089/ten.tec.2017.0090.
[20] GAFAROVA E R,GREBENIK E A,LAZHKO A E,et al.Evaluation of supercritical CO2-assisted protocols in a model of ovine aortic root decellularization[J].Molecules,2020,25(17):3923.DOI:10.3390/molecules25173923.
[21] CHUNG S,KWON H,KIM N P.Supercritical extraction of decellularized extracellular matrix from porcine adipose tissue as regeneration therapeutics[J].Journal of Cosmetic Medicine,2019,3(2):86-93.DOI:10.25056/JCM.2019.3.2.86.
[22] CASALI D M,HANDLETON R M,SHAZLY T,et al.A novel supercritical CO2-based decellularization method for maintaining scaffold hydration and mechanical properties[J].The Journal of Supercritical Fluids,2018,131:72-81.DOI:10.1016/j.supflu.2017.07.021.
[23] AFFATATO S,BORDINI B,FAGNANO C,et al.Effects of the sterilisation method on the wear of UHMWPE acetabular cups tested in a hip joint simulator[J].Biomaterials,2002,23(6):1439-1446.DOI:10.1016/s0142-9612(01)00265-4.
[24] MENDES G,BRAND?O T,SILVA C.Ethylene oxide sterilization of medical devices:A review[J].American Journal of Infection Control,2007,35(9):574-581.DOI:10.1016/j.ajic.2006.10.014.
[25] FRASER D.Bursting bacteria by release of gas pressure[J].Nature,1951,167(4236):33-34.DOI:10.1038/167033b0.
[26] ZHANG Jian,DAVIS T A,MATTHEWS M A,et al.Sterilization using high-pressure carbon dioxide[J].The Journal of Supercritical Fluids,2006,38(3):354-372.DOI:10.1016/j.supflu.2005.05.005.
[27] GARCIA-GONZALEZ L,GEERAERD A H,SPILIMBERGO S,et al.High pressure carbon dioxide inactivation of microorganisms in foods:The past,the present and the future[J].International Journal of Food Microbiology,2007,117(1):1-28.DOI::10.1016/j.ijfoodmicro.2007.02.018.
[28] SHIEH E,PASZCZYNSKI A,WAI C M,et al.Sterilization of bacillus pumilus spores using supercritical fluid carbon dioxide containing various modifier solutions[J].Journal of Microbiological Methods,2009,76(3):247-252.DOI:10.1016/j.mimet.2008.11.005.
[29] HENNESSY R S,JANA S,TEFFT B J,et al.Supercritical carbon dioxide–based sterilization of decellularized heart valves[J].JACC:Basic to Translational Science,2017,2(1):71-84.DOI:10.1016/j.jacbts.2016.08.009.
[30] 闫伽宁,胥义.脱细胞基质生物墨水制备方法及应用进展[J].生物工程学报,2021,37(11):4024-4035.DOI:10.13345/j.cjb.210091.
[31] REIS D P,DOMINGUES B,FIDALGO C,et al.Bioinks enriched with ECM components obtained by supercritical extraction[J].Biomolecules,2022,12(3):394.DOI:10.3390/biom12030394.
[32] SALDIN L T,CRAMER M C,VELANKAR S S,et al.Extracellular matrix hydrogels from decellularized tissues:Structure and function[J].Acta Biomaterialia,2017,49:1-15.DOI:10.1016/j.actbio.2016.11.068.
[33] ZHANG Ying,ZHANG Chenyu,LI Yuwen,et al.Evolution of biomimetic ECM scaffolds from decellularized tissue matrix for tissue engineering:A comprehensive review[J].International Journal of Biological Macromolecules,2023,246:125672.DOI:10.1016/j.ijbiomac.2023.125672.
[34] WANG C H,HSIEH D J,PERIASAMY S,et al.Regenerative porcine dermal collagen matrix developed by supercritical carbon dioxide extraction technology:Role in accelerated wound healing[J].Materialia,2020,9:100576.DOI:10.1016/j.mtla.2019.100576.
[35] HUANG Y H,TSENG F W,CHANG W H,et al.Preparation of acellular scaffold for corneal tissue engineering by supercritical carbon dioxide extraction technology[J].Acta Biomaterialia,2017,58:238-243.DOI:10.1016/j.actbio.2017.05.060.

备注/Memo

备注/Memo:
收稿日期: 2024-07-01
通信作者: 陈爱政(1978-),男,教授,博士,博士生导师,主要从事超临界流体技术、生物材料与组织工程的研究。E-mail:azchen@hqu.edu.cn。
基金项目: 国家自然科学基金面上资助项目(32271410)
更新日期/Last Update: 2024-09-20