[1]严慧,周晶晶,林宏峻,等.双功能型纤溶酶的研究进展[J].华侨大学学报(自然科学版),2023,44(4):427-434.[doi:10.11830/ISSN.1000-5013.202306013]
 YAN Hui,ZHOU Jingjing,LIN Hongjun,et al.Research Progress of Dual-Functional Fibrinolytic Enzyme[J].Journal of Huaqiao University(Natural Science),2023,44(4):427-434.[doi:10.11830/ISSN.1000-5013.202306013]
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双功能型纤溶酶的研究进展()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第44卷
期数:
2023年第4期
页码:
427-434
栏目:
出版日期:
2023-07-17

文章信息/Info

Title:
Research Progress of Dual-Functional Fibrinolytic Enzyme
文章编号:
1000-5013(2023)04-0427-08
作者:
严慧 周晶晶 林宏峻 唐明青
华侨大学 医学院, 福建 泉州 362021
Author(s):
YAN Hui ZHOU Jingjing LIN Hongjun TANG Mingqing
School of Medicine, Huaqiao University, Quanzhou 362021, China
关键词:
纤溶酶原 纤维蛋白 纤溶酶 纤溶 血栓
Keywords:
plasminogen fibrin fibrinolytic enzyme fibrinolysis thrombosis
分类号:
Q71
DOI:
10.11830/ISSN.1000-5013.202306013
文献标志码:
A
摘要:
综述双功能型纤溶酶(dFE)的物种来源,介绍双功能型纤溶酶的纯化工艺和酶活特性,为其进一步开发提供参考.结果表明:双功能型纤溶酶是一种同时具有纤溶酶原激活功能和纤维蛋白直接降解功能的新型纤溶物质,其来源广泛,多为微生物来源;分离纯化工艺繁琐,以多层色谱为主;大多体现丝氨酸蛋白酶活性特征,受金属离子、pH值和蛋白酶抑制剂影响较大.
Abstract:
The species origin of dual-functional fibrinolytic enzyme(dFE)was reviewed,and the purification processes and enzyme activity characteristics of dFE were introduced to provide reference for its further development. The results showed that dFE was a novel fibrinolytic agent with both the functions of plasminogen activation and direct fibrinolysis. It had a wide range of sources, mostly microorganisms sources. The separation and purification processes were complicated, mainly based on multi-layer chromatography. It mostly exhibited the enzymatic characteristics of serine proteinase,and was greatly affected by metal ions, pH value and protease inhibitors.

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备注/Memo

备注/Memo:
收稿日期: 2023-06-15
通信作者: 唐明青(1982-),男,副教授,博士,主要从事血栓纤溶系统及基因治疗的研究.E-mail:Tmq@hqu.edu.cn.
基金项目: 福建省科技计划项目(2019J01070); 福建省泉州市科技计划项目(2018Z012); 福建省厦门市科技计划项目(3502Z20227197); 福建省中青年教师教育科研项目(JA14020); 华侨大学人才引进项目(14BS111)
更新日期/Last Update: 2023-07-20