[1]张雨凝,郑茗冉,贾海强,等.雨生红球藻油柔性纳米脂质体冻干粉的制备与评价[J].华侨大学学报(自然科学版),2022,43(6):769-776.[doi:10.11830/ISSN.1000-5013.202204005]
　ZHANG Yuning,ZHENG Mingran,JIA Haiqiang,et al.Preparation and Evaluation of Flexible Nano Liposome Lyophilized Powder of Haematococcus Pluvialis Oil[J].Journal of Huaqiao University(Natural Science),2022,43(6):769-776.[doi:10.11830/ISSN.1000-5013.202204005]
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雨生红球藻油柔性纳米脂质体冻干粉的制备与评价()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第43卷
期数:
2022年第6期
页码:
769-776
栏目:
出版日期:
2022-11-11

文章信息/Info

Title:
Preparation and Evaluation of Flexible Nano Liposome Lyophilized Powder of Haematococcus Pluvialis Oil
文章编号:
1000-5013(2022)06-0769-08
作者:
张雨凝1;  郑茗冉1;  贾海强1;  吴振2;  刘智阳3;  王立强1
1. 华侨大学 医学院, 福建 泉州 362021;2. 厦门大学 药学院, 福建 厦门361100;3. 贵州中铭生物科技有限公司, 贵州 贵阳 550001
Author(s):
ZHANG Yuning1;  ZHENG Mingran1;  JIA Haiqiang1; WU Zhen2;  LIU Zhiyang3;  WANG Liqiang1
1. School of Medicine, Huaqiao University, Quanzhou 362021, China; 2. College of Pharmacy, Xiamen University, Xiamen 361000, China; 3. Guizhou Zhongming Biotechnology Limited Company, Guiyang 550001, China
关键词:
雨生红球藻油;  柔性纳米脂质体;  冻干粉;  体外评价;  药动学
Keywords:
haematococcus pluvialis oil;  flexible nano liposome;  lyophilized powder;  in vitro evaluation;  pharmacokinetics
分类号:
Q949.212.05;R94
DOI:
10.11830/ISSN.1000-5013.202204005
文献标志码:
A
摘要:
采用熔融乳化法与冷冻干燥法制备雨生红球藻油柔性纳米脂质体冻干粉(Hpo-FNL-LP),并研究其包封率、载药量、粒径、Zeta电位、微观形态及稳定性.采用桨法测定Hpo-FNL-LP体外累积溶出率,扩散池法测定皮肤累计渗透量,高效液相色谱法(HPLC)法测定虾青素血药质量浓度.结果表明:Hpo-FNL-LP的包封率为(89.16±1.21)%,载药量为(1.34±0.04)%,粒径为(165.75±0.11)nm,Zeta电位为(-18.45±0.48)mV,微观形态为双层膜的圆形结构,稳定性较好;与雨生红球藻油乙醇溶液相比,Hpo-FNL-LP具有一定的缓释效果;Hpo-FNL-LP中虾青素24 h的皮肤累计渗透量达到(12.84±0.36)μg·cm-2,显著高于雨生红球藻油普通脂质体;与虾青素油溶液相比,Hpo-FNL-LP的t1/2延长,ρmax与AUC0-t增大,相对生物利用度高达(422.53±3.30)%.
Abstract:
The flexible nano liposome lyophilized powder of haematococcus pluvialis oil(Hpo-FNL-LP)was prepared by melt emulsification method and lyophilization method, and the encapsulation rate, drug loading amount, particle size, Zeta potential, microscopic morphology and stability were investigated. The cumulative dissolution rate of Hpo-FNL-LP in vitro was determined by paddle method. The cumulative skin penetration amount was determined by diffusion chamber method. The blood mass concentration of astaxanthin was determined by high performance liquid chromatography(HPLC)method. The results show that, the encapsulation rate of Hpo-FNL-LP is(89.16±1.21)%, the drug loading amount is(1.34±0.04)%, the particle size is(165.75±0.11)nm, and the Zeta potential is(-18.45±0.48)mV. The microscopic morphology shows a double-layer circular structure with good stability. Compared with haematococcus pluvialis oil ethanol solution, Hpo-FNL-LP has a certain sustained release effect. The cumulative penetration amount of astaxanthin in Hpo-FNL-LP at 24 h reaches(12.84±0.36)μg·cm-2, which is significantly higher than that of haematococcus pluvialis oil ordinary liposome. Compared with the astaxanthin oil solution, the t1/2 of Hpo-FNL-LP extends,ρmax and AUC0-t both increase, and the relative bioavailability rate is as high as(422.53±3.30)%.

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备注/Memo

备注/Memo:
收稿日期: 2022-04-05
通信作者: 王立强(1970-),男,博士,教授,主要从事药剂学和创新药物的研究.E-mail:wlq1599@163.com.
基金项目: 国家重点研发计划项目(2016YFE0101700); 福建省高校产学合作重大项目(2019Y4007)http://www.hdxb.hqu.edu.cn
更新日期/Last Update: 2022-11-20