[1]王晓,黄晓平,黎玲,等.重组腺相关病毒基因药物三种滴度的比较与分析[J].华侨大学学报(自然科学版),2021,42(4):507-511.[doi:10.11830/ISSN.1000-5013.202010006]
　WANG Xiao,HUANG Xiaoping,LI Ling,et al.Comparison and Analysis of Three Titers of Recombinant Adeno-Associated Virus Gene Drugs[J].Journal of Huaqiao University(Natural Science),2021,42(4):507-511.[doi:10.11830/ISSN.1000-5013.202010006]
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重组腺相关病毒基因药物三种滴度的比较与分析()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第42卷
期数:
2021年第4期
页码:
507-511
栏目:
出版日期:
2021-07-20

文章信息/Info

Title:
Comparison and Analysis of Three Titers of Recombinant Adeno-Associated Virus Gene Drugs
文章编号:
1000-5013(2021)04-0507-05
作者:
王晓1;  黄晓平2;  黎玲1;  刁勇1
1. 华侨大学 医学院, 福建 泉州 362021;2. 泉州师范学院 化工与材料学院, 福建 泉州 362000
Author(s):
WANG Xiao1;  HUANG Xiaoping2;  LI Ling1;  DIAO Yong1
1. School of Medicine, Huaqiao University, Quanzhou 362021, China; 2. College of Chemical Engineering and Materials Sciences, Quanzhou Normal University, Quanzhou 362000, China
关键词:
重组腺相关病毒;  衣壳滴度;  基因组滴度;  转导滴度;  基因治疗
Keywords:
recombinant adeno-associated virus;  particle titer;  genome titer;  transduction titer;  gene therapy
分类号:
R446.6;R394.6
DOI:
10.11830/ISSN.1000-5013.202010006
文献标志码:
A
摘要:
采用酶联免疫吸附测定(ELISA)、实时荧光定量聚合酶链反应(Q-PCR)和转导方法对重组腺相关病毒(rAAV)的衣壳滴度、基因组滴度、转导滴度进行定量,并比较衣壳滴度/基因组滴度(P/GC)和基因组滴度/转导滴度(GC/TU).实验结果表明:3批rAAV的平均衣壳滴度为3.65×1013 P·mL-1,平均基因组滴度为8.67×1011 GC·mL-1,平均转导滴度为9.85×109 TU·mL-1,P/GC平均值为41.70,GC/TU平均值为88.20,P/GC和GC/TU平均值接近于AAV2标准品,说明文中方法的制备工艺成熟、稳定,制备的rAAV感染活性较高.
Abstract:
The particle titer, genome titer and transduction titer of recombinant adeno-associated virus(rAAV)were quantified by enzyme-linked immuno sorbent assay(ELISA), quantitative real-time polymerase chain reaction(Q-PCR)and transduction method, and then the particle titer/genome titer(P/GC)and genome titer/transduction titer(GC/TU)were compared. The experiment results showed that the average particle titer of rAAV in three batches was 3.65×1013 P·mL-1, the average genome titer was 8.67×1011 GC·mL-1, the average transduction titer was 9.85×109 TU·mL-1, the average value of P/GC was 41.70 and the average value of GC/TU was 88.20. The average values of P/GC and GC/TU were close to the AAV2 standard, indicating that the preparation process of the method in this paper is mature and stable, and the prepared rAAV has high infectious activity.

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相似文献/References:

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　XIAO Gui-qing,YANG Hui-yong,DIAO Yong.Advances in Real-Time Quantitative PCR Technology for Quality Control of Recombinant Adeno-Associated Virus[J].Journal of Huaqiao University(Natural Science),2014,35(4):191.[doi:10.11830/ISSN.1000-5013.2014.02.0191]
[2]唐明青,张文豪,侯莹,等.超滤法去除重组腺相关病毒中内毒素的应用[J].华侨大学学报(自然科学版),2017,38(1):64.[doi:10.11830/ISSN.1000-5013.201701012]
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备注/Memo

备注/Memo:
收稿日期: 2020-10-08
通信作者: 刁勇(1967-),男,教授,博士,博士生导师,主要从事基因药物的研究.E-mail:diaoyong@hqu.edu.cn.
基金项目: 国家自然科学基金资助项目(81371669); 福建省自然科学基金资助项目(2017J01548); 福建省泉州市科技计划项目(2020C061, 2016N070); 华侨大学科研基金资助项目(17BS501)
更新日期/Last Update: 2021-07-20