[1]刘青,李永,盛世美.金线莲多糖抗衰老作用及其机制[J].华侨大学学报(自然科学版),2020,41(1):77-83.[doi:10.11830/ISSN.1000-5013.201907059]
　LIU Qing,LI Yong,SHENG Shimei.Anti-Aging Effects and Mechanisms of Anoectochilus roxburghii Polysaccharose[J].Journal of Huaqiao University(Natural Science),2020,41(1):77-83.[doi:10.11830/ISSN.1000-5013.201907059]
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金线莲多糖抗衰老作用及其机制()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第41卷
期数:
2020年第1期
页码:
77-83
栏目:
出版日期:
2020-01-20

文章信息/Info

Title:
Anti-Aging Effects and Mechanisms of Anoectochilus roxburghii Polysaccharose
文章编号:
1000-5013(2020)01-0077-07
作者:
刘青;  李永;  盛世美
华侨大学 化工学院, 福建 厦门 361021
Author(s):
LIU Qing;  LI Yong;  SHENG Shimei
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
金线莲多糖;  NF-κB信号通路;  抗氧化;  认知能力;  抗衰老
Keywords:
Anoectochilus roxburghii polysaccharose;  NF-κB signaling pathway;  antioxidant;  cognitive ability;  anti-aging
分类号:
R285;S567
DOI:
10.11830/ISSN.1000-5013.201907059
文献标志码:
A
摘要:
考察金线莲多糖抗衰老作用及其机制.每天给小鼠皮下注射D-半乳糖溶液(40 mg·kg-1)诱导其衰老,同时灌胃给予金线莲多糖(100,200,300 mg·kg-1)或维生素E(200 mg·kg-1)进行治疗,以维生素E治疗组为阳性对照.6周后对小鼠进行行为学测试,8周后检测小鼠的大脑皮层p-NF-κB p65表达,以及大脑皮层过氧化氢酶、超氧化物歧化酶及血清总抗氧化能力活性和丙二醛浓度,并检测小鼠腹腔巨噬细胞的吞噬情况.结果表明:与衰老小鼠相比较,经金线莲多糖治疗后,小鼠的抗氧化酶活性显著提高,其大脑皮层p-NF-κB p65表达下调,且运动、空间探索和学习记忆能力明显改善;同时,金线莲多糖能增强衰老小鼠清除抗原的能力;金线莲多糖抗衰老作用与其抗氧化、抑制NF-κB信号通路、增强衰老小鼠免疫能力有关.
Abstract:
The anti-aging effect and mechanisms of Anoectochilus roxburghii polysaccharose(ARP)was studied. The aging mouse model was induced by subcutaneous injection of D-galactose solution(40 mg·kg-1)daily, then the treated mice were intragastrically administered with ARP(100, 200 and 300 mg·kg-1)or Vitamin E(200 mg·kg-1), and Vitamin E treatment group was used as a positive control. After 6 weeks, the behavioral tests were performed. All mice were sacrificed after 8 weeks, and the expression of p-NF-κB p65 and antioxidant enzymes including superoxide dismutase and catalase in the cerebral cortex were examined. The activity of total antioxidant enzyme activity and malondialdehyde content in serum were measured and the phagocytic ability of mouse peritoneal macrophages were detected. The results showed that compared with aging mice, treatment with ARP could increase the activity of antioxidant enzymes significantly and reduce p-NF-κB p65 expression in cerebral cortex, and increase the movement abilities, space exploration, learning and memory abilities of aging mice. Meanwhile, ARP could enhance the ability of aging mice to clear antigen. The ARP anti-aging effect was due to its antioxidant effect, the inhibition of NF-κB signaling pathway and the improved immunity.

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相似文献/References:

[1]刘青,刘珍伶,周娟.金线莲多糖的体外抗氧化活性[J].华侨大学学报(自然科学版),2010,31(6):718.[doi:10.11830/ISSN.1000-5013.2010.06.0718]
　LIU Qing,LIU Zhen-ling,ZHOU Juan.Study on Antioxidative Activity of Anoectochilus formosams Polysaccharide in Vitro[J].Journal of Huaqiao University(Natural Science),2010,31(1):718.[doi:10.11830/ISSN.1000-5013.2010.06.0718]
[2]盛世美,张建刚,陈烨,等.金线莲多糖通过抑制NF-κB信号通路对糖尿病小鼠肾损伤的改善作用[J].华侨大学学报(自然科学版),2024,45(5):635.[doi:10.11830/ISSN.1000-5013.202402003]
　SHENG Shimei,ZHANG Jiangang,CHEN Ye,et al.Improvement Effects of Anoectochilus roxburghii Polysaccharose on Renal Injury in Diabetic Mice by Inhibiting NF-кB Signaling Pathway[J].Journal of Huaqiao University(Natural Science),2024,45(1):635.[doi:10.11830/ISSN.1000-5013.202402003]

备注/Memo

备注/Memo:
收稿日期: 2019-07-22
通信作者: 刘青(1970-),女,副教授,博士,主要从事天然药物的提取及活性的研究.E-mail:yunliu@126.com.
基金项目: 福建省自然科学基金资助项目(2013J01337)
更新日期/Last Update: 2020-01-20