[1]杨道茂,陈煌.近海海洋真菌生物转化(+)-柠檬烯[J].华侨大学学报(自然科学版),2020,41(1):67-71.[doi:10.11830/ISSN.1000-5013.201904020]
　YANG Daomao,CHEN Huang.Biotransformation of(+)-Limoneneby Offshore Marine Fungi[J].Journal of Huaqiao University(Natural Science),2020,41(1):67-71.[doi:10.11830/ISSN.1000-5013.201904020]
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近海海洋真菌生物转化(+)-柠檬烯()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第41卷
期数:
2020年第1期
页码:
67-71
栏目:
出版日期:
2020-01-20

文章信息/Info

Title:
Biotransformation of(+)-Limoneneby Offshore Marine Fungi
文章编号:
1000-5013(2020)01-0067-05
作者:
杨道茂1;  陈煌2
1. 华侨大学 化工学院, 福建 厦门 361021;2. 厦门万泰沧海生物技术有限公司, 福建 厦门 361022
Author(s):
YANG Daomao1;  CHEN Huang2
1. College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2. Xiamen Innovax Biotech Limited Company, Xiamen 361022, China
关键词:
(+)-柠檬烯;  海洋真菌;  生物转化;  液体发酵法;  异辣薄荷酮
Keywords:
(+)-limonene;  marine fungi;  biotransformation;  liquid fermentation method;  isopiperitenone
分类号:
Q939.9;Q949.321
DOI:
10.11830/ISSN.1000-5013.201904020
文献标志码:
A
摘要:
以厦门近海海域分离到的30株海洋真菌为出发菌株,通过液体发酵法考察其生物转化(+)-柠檬烯的能力,采用气相色谱-质谱(GC-MS)技术结合NIST数据库对其代谢产物进行化合物分析.结果表明:23,27,36,41号真菌具有生物转化柠檬烯的能力,其主要代谢产物暂定为异辣薄荷烯酮、波斯菊萜、1,3,8-孟三烯、香芹醇等;在23,36号真菌的代谢产物中,异辣薄荷烯酮为唯一主要产物,具有进一步研究开发的价值.
Abstract:
Thirty marine fungi isolated from Xiamen City offshore district were used as starting strains to investigate their ability to biotransform(+)-limonene by liquid fermentation method, and their metabolites were analyzed by gas chromatography-mass spectrometry(GC-MS)combined with NIST database. The results showed that strain 23, 27, 36 and 41 had the biotransformation ability of limonene. The main metabolites were tentatively identified as isopiperitenone, cosmene, 1,3,8-menthatriene, carveol, etc. The isopiperitenone was the only major product among metabolites produced from strain 23 and 36, which is valuable for further research and exploitation.

参考文献/References:

[1] MANTZOURIDOU F T,PARASKEVOPOULOU A,LALOU S.Yeast flavour production by solid state fermentation of orange peel waste[J].Biochemical Engineering Journal,2015,101:1-8.DOI:10.1016/j.bej.2015.04.013.
[2] VESPERMANN K A C,PAULINO B N,BARCELOS M C S,et al.Biotransformation of α- and β-pinene into flavor compounds[J].Applied Microbiology and Biotechnology,2017,101(5):1805-1817.DOI:10.1007/s00253-016-8066-7.
[3] CARROLL A L,DESAI S H,ATSUMI S.Microbial production of scent and flavor compounds[J].Current Opinion Biotechnology,2016,37:8-15.DOI:10.1016/j.copbio.2015.09.003.
[4] van der WERF M J,SWARTS H J,de BONT J A M.Rhodococcus erythropolis DCL14 contains a novel degradation pathway for limonene[J].Applied and Environmental Microbiology,1999,65(5):2092-2102.DOI:10.1128/AEM.65.5.2092-2102.1999.
[5] MAROSTICA M R,PASTORE G M.Biotransformation of limonene: A review of the main metabolic pathways[J].Quimica Nova,2007,30(2):382-387.DOI:10.1590/S0100-40422007000200027.
[6] BIER M C J,MEDEIROS A B P,SOCCOL C R.Biotransformation of limonene by an endophytic fungus using synthetic and orange residue-based media[J].Fungal Biology,2017,121(2):137-144.DOI:10.1016/j.funbio.2016.11.003.
[7] RASOUL-AMINI S,FOTOOH-ABADI E,GHASEMI Y.Biotransformation of monoterpenes by immobilized microalgae[J].Journal of Applied Phycology,2011,23(6):975-981.DOI:10.1007/s10811-010-9625-4.
[8] BICAS J L,de QUADROS C P,NERI-NUMA I A,et al.Integrated process for co-production of alkaline lipase and R-(+)-α-terpineol by Fusarium oxysporum[J].Food Chemistry,2010,120(2):452-456.DOI:10.1016/j.foodchem.2009.10.037.
[9] BICAS J L,BARROS F F C,WAGNER R,et al.Optimization of R-(+)-α-terpineol production by the biotransformation of R-(+)-limonene[J].Journal of Industrial Microbiology and Biotechnology,2008,35(9):1061-1070.DOI:10.1007/s10295-008-0383-0.
[10] TAI Yanan,XU Min,REN Jingnan,et al.Optimisation of α-terpineol production by limonene biotransformation using Penicillium digitatum DSM 62840[J].Journal of the Science of Food and Agriculture,2016,96(3):954-961.DOI:10.1002/jsfa.7171.
[11] BADEE A Z M,HELMY S A,MORSY N F S.Utilisation of orange peel in the production of α-terpineol by Penicillium digitatum(NRRL 1202)[J].Food Chemistry,2011,126(3):849-854.DOI:10.1016/j.foodchem.2010.11.046.
[12] BICAS J L,FONTANILLE P,PASTORE G M,et al.Characterization of monoterpene biotransformation in two pseudomonads[J].Journal of Applied Microbiology,2008,105(6):1991-2001.DOI:10.1111/j.1365-2672.2008.03923.x.
[13] FERRARA M A,ALMEIDA D S,SIANI A C,et al.Bioconversion of R-(+)-limonene to perillic acid by the yeast Yarrowia lipolytica[J].Brazilian Journal of Microbiology,2013,44(4):1075-1080.DOI:10.1590/S1517-83822014 005000008.
[14] van RENSBURG E,MOLELEKI N,van der WALI J P,et al.Biotransformation of(+)-limonene and(-)-piperitone by yeasts and yeast-like fungi[J].Biotechnology Letters,1997,19(8):779-782.DOI:10.1023/A:101834 4411069.
[15] MOLINA G,BUTION M L,BICAS J L,et al.Comparative study of the bioconversion process using R-(+)-and S-(-)-limonene as substrates for Fusarium oxysporum 152B[J].Food Chemistry,2015,174(1):606-613.DOI:10.1016/j.foodchem.2014.11.059.
[16] SCHWAB W,FUCHS C,HUANG F C.Transformation of terpenes into fine chemicals[J].European Journal of Lipid Science and Technology,2013,115(1):3-8.DOI:10.1002/ejlt.201200157.
[17] ROTTAVA I,CORTINA P F,GRANDO C E,et al.Isolation and screening of microorganisms for R-(+)-limonene and(-)-β-pinene biotransformation[J].Applied Biochemistry and Biotechnology,2010,162(3):719-732.DOI:10.1007/s12010-009-8872-9.
[18] BICAS J L,PASTORE G M.Isolation and screening of D-limonene-resistant microorganisms[J].Brazilian Journal of Microbiology,2007,38(3):563-567.DOI:10.1590/S1517-83822007000300034.
[19] 牟海津.海洋微生物工程[M].青岛:中国海洋大学出版社,2016.
[20] CHATTERJEE T,BHATTACHARYYA D K.Biotransformation of limonene by Pseudomonas putida[J].Applied Microbiology and Biotechnology,2001,55(5):541-546.DOI:10.1007/s002530000538.
[21] MIRATA M A,HEERD D,SCHRADER J.Integrated bioprocess for the oxidation of limonene to perillic acid with Pseudomonas putida DSM 12264[J].Process Biochemistry,2009,44(7):764-771.DOI:10.1016/j.procbio.2009.03.013.
[22] van BEILEN J B,HOLTACKERS R,LUSCHER D,et al.Biocatalytic production of perillyl alcohol from limonene by using a novel Mycobacterium sp. cytochrome P450 alkane hydroxylase expressed in Pseudomonas putida[J].Applied and Environmental Microbiology,2005,71(4):1737-1744.DOI:10.1128/AEM.71.4.1737-1744.2005.
[23] LI Houjin,LAN Wenjian,CAI Chuanghua,et al.Biotransformation of limonene by marine bacteria[J].Chinese Journal of Analytical Chemistry,2006,34(7):946-950.DOI:10.1016/S1872-2040(06)60046-7.
[24] MIYAZAWA M,WADA T,KAMEOKA H.Biotransformation of(+)- and(-)-limonene by the larvae of common cutworm(Spodoptera litura)[J].Journal of Agricultural and Food Chemistry,1998,46(1):300-303.DOI:10.1021/jf970142l.

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备注/Memo

备注/Memo:
收稿日期: 2019-04-11
通信作者: 杨道茂(1975-),男,讲师,博士,主要从事生物转化及天然生物活性物质分离的研究.E-mail:ydmao@hqu.edu.cn.
基金项目: 华侨大学高层次人才科研启动基金资助项目(Z15X0004)
更新日期/Last Update: 2020-01-20