[1]吴思晓,周玥莹,于慧敏,等.反式白藜芦醇纳米粒的制备及其体外评价[J].华侨大学学报(自然科学版),2019,40(6):771-778.[doi:10.11830/ISSN.1000-5013.201903039]
　WU Sixiao,ZHOU Yueying,YU Huimin,et al.Preparation and In Vitro Evaluation of Trans-Resveratrol Nanoparticles[J].Journal of Huaqiao University(Natural Science),2019,40(6):771-778.[doi:10.11830/ISSN.1000-5013.201903039]
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反式白藜芦醇纳米粒的制备及其体外评价()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第40卷
期数:
2019年第6期
页码:
771-778
栏目:
出版日期:
2019-11-20

文章信息/Info

Title:
Preparation and In Vitro Evaluation of Trans-Resveratrol Nanoparticles
文章编号:
1000-5013(2019)06-0771-08
作者:
吴思晓1;  周玥莹1;  于慧敏2;  王立强1
1. 华侨大学 生物医学学院, 福建 泉州 362021;2. 哈尔滨医科大学 附属第二医院, 黑龙江 哈尔滨 150080
Author(s):
WU Sixiao1;  ZHOU Yueying1;  YU Huimin2;  WANG Liqiang1
1. School of Biomedical Sciences, Huaqiao University, Quanzhou 362021, China; 2. Second Affiliated Hospital, Harbin Medical University, Harbin 150080, China
关键词:
白藜芦醇;  纳米粒;  Box-Behnken效应面法;  工艺优化;  体外评价
Keywords:
resveratrol;  nanoparticles;  Box-Behnken effect surface method;  process optimization;  in vitro evaluation
分类号:
R285;R931
DOI:
10.11830/ISSN.1000-5013.201903039
文献标志码:
A
摘要:
采用离子交联法制备反式白藜芦醇纳米粒(t-Res-NPs),通过Box-Behnken效应面法优化制备工艺.从包封率、粒径、Zeta电位、载药量、纳米粒形态、缓释作用、稳定性等方面对t-Res-NPs进行体外评价.结果表明:t-Res-NPs粒径为(85.38±1.69)nm,Zeta电位为(19.93±3.25)mV,包封率为(88.31±0.59)%,载药量为(5.96±1.60)%;纳米粒形态呈圆形;t-Res-NPs具有良好的缓释作用,释放过程较为平稳,突释现象不明显;肠内菌对t-Res-NPs及反式白藜芦醇(t-Res)几乎无代谢作用,肝脏代谢酶对t-Res具有强烈的代谢作用,而t-Res-NPs可以有效地保护药物,减慢其代谢速率;t-Res-NPs可明显改善t-Res溶解度差、生物利用度低的缺点.
Abstract:
Trans-resveratrol nanoparticles(t-Res-NPs)were prepared by ion-crosslinking, and the preparation process was optimized by Box-Behnken effect surface method. t-Res-NPs were evaluated in vitro from the aspects of encapsulation efficiency, particle size, Zeta potential, drug loading, nanoparticle morphology, sustained release and stability. The results show that the prepared t-Res-NPs have particle size of(85.38±1.69)nm, Zeta potential of(19.93±3.25)mV, entrapment efficiency of(88.31±0.59)%, and the drug loading of(5.96±1.60)%; the nanoparticle morphology is round; t-Res-NPs have a good sustained release effect, and the release process is relatively stable, which has no obvious burst release phenomenon; intestinal bacteria have almost no metabolism to t-Res-NPs and trans-resveratrol(t-Res), while liver metabolic enzymes have a strong metabolic effect on t-Res, and t-Res-NPs can protect the drug effectively and slow down its metabolic rate; t-Res-NPs can improve the poor solubility and low bioavailability of t-Res significantly.

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备注/Memo

备注/Memo:
收稿日期: 2019-03-18
通信作者: 王立强(1970-),男,教授,博士,主要从事药剂学和创新药物的研究.E-mail:wlq1599@163.com.
基金项目: 国家重点研发计划项目(2016YFE0101700); 福建省高校产学合作项目(2019Y4007)
更新日期/Last Update: 2019-11-20