[1]江伟,裴蕊,胡鹏程,等.具有合成(S)-度洛西汀潜力的功能菌株高效筛选及检测分析[J].华侨大学学报(自然科学版),2019,40(3):370-375.[doi:10.11830/ISSN.1000-5013.201811010]
　JIANG Wei,PEI Rui,HU Pengcheng,et al.Efficient Screening and Analysis of Functional Strains With Potential to Synthesize(S)-Duloxetine[J].Journal of Huaqiao University(Natural Science),2019,40(3):370-375.[doi:10.11830/ISSN.1000-5013.201811010]
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具有合成(S)-度洛西汀潜力的功能菌株高效筛选及检测分析()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第40卷
期数:
2019年第3期
页码:
370-375
栏目:
出版日期:
2019-05-20

文章信息/Info

Title:
Efficient Screening and Analysis of Functional Strains With Potential to Synthesize(S)-Duloxetine
文章编号:
1000-5013(2019)03-0370-06
作者:
江伟1; 2;  裴蕊1; 2;  胡鹏程1; 2;  钟丽娟1; 2;  周树锋1; 2
1. 华侨大学 福建省生物化工技术重点实验室, 福建 厦门 361021;2. 华侨大学 化工学院, 福建 厦门 361021
Author(s):
JIANG Wei1; 2;  PEI Rui1; 2;  HU Pengcheng1; 2;  ZHONG Lijuan1; 2;  ZHOU Shufeng1; 2
1. Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen 361021, China; 2. College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
(S)-度洛西汀;  生物催化;  手性药物;  高效筛选;  Bacillus megaterium;  抑郁症
Keywords:
(S)-duloxetine;  biocatalysis;  chiral drugs;  efficient screening;  Bacillus megaterium;  depression
分类号:
Q939.97
DOI:
10.11830/ISSN.1000-5013.201811010
文献标志码:
A
摘要:
提出一种高效筛选具有催化合成(S)-度洛西汀功能菌株的方法.以来源于福建省厦门市附近海域的海水及淤泥中的微生物组为出发材料,经过菌株培养、富集及贫瘠筛选培养基筛选,梯度补加苯乙酮,利用细胞催化降解苯乙酮,并进行平板筛选和16S rDNA鉴定,从而获得一株可以高效利用苯乙酮的功能菌株Bacillus megaterium(B. megaterium).利用静息细胞法和核磁共振技术进行检测和验证,结果表明:B. megaterium具有催化N,N-二甲基-3-酮-3-(2-噻吩)-1-丙胺(DKTP)不对称转化制备(S)-N,N-二甲基-3-羟基-3-(2-噻吩)-1-丙胺(DHTP)的潜在能力.
Abstract:
An efficient method to screen the functional strain with catalytic synthesis(S)-duloxetine was established. The microbiome which was obtained from sea water and silt near Xiamen City, Fujian Province was selected as the start material. Strain cultivation, enrichment and sterile filter medium in the absence of energy source, gradient addition acetophenone for gradually increasing the concentration of acetophenone, catalytic degradation of acetophenone using cell, plate screening and 16S rDNA were selected to screen functional strain which can use acetophenone efficiently, and finally the Bacillus megaterium(B. megaterium)was obtained. Detection and verification by resting cell method and nuclear magnetic resonance technology, the results show that B. megaterium has potential to catalyze asymmetric transformation of N,N-dimethyl-3-keto-3-(2-thienyl)-1-propa-namine(DKTP)to(S)-N,N-dimethyl-3-hydroxy-3-(2-thienyl)-1-propanamine(DHTP).

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备注/Memo

备注/Memo:
收稿日期: 2018-11-07
通信作者: 江伟(1987-),男,讲师,博士,主要从事合成生物学与生物信息学、生物催化与生物转化、应用酶学与定向进化的研究.E-mail:wjiang@hqu.edu.cn.
基金项目: 福建省泉州市科技计划项目(2018C008); 华侨大学高层次人才科研启动项目(600005-Z17Y0072)
更新日期/Last Update: 2019-05-20