[1]曾皓月,王之,方美娟,等.pDNA-巯基壳聚糖纳米粒的制备及Box-Behnken效应面法工艺优化[J].华侨大学学报(自然科学版),2017,38(5):676-681.[doi:10.11830/ISSN.1000-5013.201703054]
　ZENG Haoyue,WANG Zhi,FANG Meijuan,et al.Preparation of pDNA-Loaded Thiolated Chitosan Nanoparticles and Optimization by Using Box-Behnken Design and Response Surface Method[J].Journal of Huaqiao University(Natural Science),2017,38(5):676-681.[doi:10.11830/ISSN.1000-5013.201703054]
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pDNA-巯基壳聚糖纳米粒的制备及Box-Behnken效应面法工艺优化()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第38卷
期数:
2017年第5期
页码:
676-681
栏目:
出版日期:
2017-09-20

文章信息/Info

Title:
Preparation of pDNA-Loaded Thiolated Chitosan Nanoparticles and Optimization by Using Box-Behnken Design and Response Surface Method
文章编号:
1000-5013(2017)05-0676-06
作者:
曾皓月1;  王之2;  方美娟3;  王立强1
1. 华侨大学 生物医学学院, 福建 泉州 362021;2. 兰州军区总医院 临床药学科, 甘肃 兰州 730000;3. 厦门大学 药学院, 福建 厦门 361000
Author(s):
ZENG Haoyue1;  WANG Zhi2;  FANG Meijuan3;  WANG Liqiang1
1. School of Biomedical Sciences, Huaqiao University, Quanzhou 362021, China; 2. Department of Clinical Pharmacy, General Hospital of Lanzhou Military Command, Lanzhou 730000, China; 3. School of Pharmaceutical Sciences, Xiamen University, Xianmen 361000,
关键词:
巯基壳聚糖;  质粒DNA;  纳米粒;  Box-Behnken效应面法;  离子交联法;  工艺优化
Keywords:
thiolated chitosan;  plasmid DNA;  nanoparticle;  Box-Behnken design and response surface method;  ionic cross-linking method;  formula optimization
分类号:
R944
DOI:
10.11830/ISSN.1000-5013.201703054
文献标志码:
A
摘要:
以巯基壳聚糖(TCS)为基因载体,采用离子交联法制备能用于基因口服研究的质粒DNA-巯基壳聚糖纳米粒(pDNA-TCS-NPs).分别以TCS质量浓度、三聚磷酸钠(TPP)质量浓度、pH值和转速为考察对象,以pDNA-TCS-NPs粒径和Zeta电位为评价指标,采用4因素3水平Box-Behnken 效应面法筛选最佳制备工艺,并对其外观形态,包封率等体外性质进行考察.结果表明:TCS质量浓度为0.80 mg·mL-1,TPP质量浓度为0.65 mg·mL-1,pH=5.3,转速为2 000 r·min-1是最优制备工艺,可制得粒径为(134.21±1.34)nm,Zeta电位为(24.36±0.29)mV,包封率在(80.26±0.56)%,形状规则且分散良好的pDNA-巯基壳聚糖纳米粒;Box-Behnken 实验设计可用于预测和优化pDNA-TCS-NP制备工艺优化筛选.
Abstract:
Preliminary exploration to prepare plasmid DNA-loaded thiolated chitosan nanoparticles(pDNA-TCS-NPs)which can be used for gene oral administration was investigated. Using TCS as gene carriers, the pDNA-TCS-NPs were prepared by the ionic cross-linking method. The effects of the concentrations of TCS and TPP, pH value and stirring speed were investigated on the particle diameter and zeta potential of the pDNA-TCS-NPs. In addition, the formula was optimized by Box-Behnken design and response surface method with four factors and three levels, and the physic-chemical properties such as the shape and encapsulation efficiency were also studied. The results showed that the optimal formula was as follows: TCS concentration 0.80 mg·mL-1, TPP concentration 0.65 mg·mL-1, pH 5.3, and stirring speed 2 000 r·min-1. Particle diameter was(134.21±1.34)nm, zeta potential was(24.36±0.29)mV, and efficiency was(80.26±0.56)%. under the optimal conditions. The pDNA-TCS-NPs showed uniform spherical solid particles with regular shape and ideal uniformly dispersion. It’s also confirmed that the Box-Behnken experimental design and response surface method could be used to predict and optimize the pDNA-loaded TCS nanoparticles.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期: 2017-03-17
通信作者: 王立强(1970-),男,教授,博士,主要从事药剂学和药物开发的研究.E-mail:wlq1599@163.com.
基金项目: 国家自然科学基金资助项目(81302652); 福建省自然科学基金资助项目(2015J01342)
更新日期/Last Update: 2017-09-20