[1]赵艳玲,周利建.巨尾桉胞质EuCuZnSOD基因的克隆与原核表达[J].华侨大学学报(自然科学版),2015,36(6):693-697.[doi:10.11830/ISSN.1000-5013.2015.06.0693]
　ZHAO Yanling,Zhou Lijian.Cloning and Expression of the Cytosolic Copper/Zinc Superoxide Dismutase Gene in Eucalyptus grandis×E. ophylla[J].Journal of Huaqiao University(Natural Science),2015,36(6):693-697.[doi:10.11830/ISSN.1000-5013.2015.06.0693]
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巨尾桉胞质EuCuZnSOD基因的克隆与原核表达()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第36卷
期数:
2015年第6期
页码:
693-697
栏目:
出版日期:
2015-11-10

文章信息/Info

Title:
Cloning and Expression of the Cytosolic Copper/Zinc Superoxide Dismutase Gene in Eucalyptus grandis×E. ophylla
文章编号:
1000-5013(2015)06-0693-05
作者:
赵艳玲;  周利建
华侨大学 化工学院, 福建 厦门 361021
Author(s):
ZHAO Yanling;  Zhou Lijian
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
巨尾桉;  EuCuZnSOD基因;  铜锌超氧化物歧化酶;  克隆;  原核表达
Keywords:
eucalyptus grandis;  copper/Zinc-superoxide dismutases;  clone;  prokaryotic expression
分类号:
Q943.2
DOI:
10.11830/ISSN.1000-5013.2015.06.0693
文献标志码:
A
摘要:
通过克隆巨尾桉的细胞质EuCuZnSOD基因,构建原核表达载体pET-CuZnSOD,并研究该基因的功能.测序结果表明:基因序列长度为459 bp;152个编码氨基酸,蛋白相对分子质量为15.2 ku.在28 ℃条件下,1 mmol·L-1 异丙基硫代半乳糖苷(IPTG)诱导4 h,转化菌株的超氧化物歧化酶(SOD),总酶活比对照组平均高19.9%.结果表明:克隆获得的巨尾桉细胞质EuCuZnSOD的重组基因表达产物具有SOD酶活性,GenBank注册号为JX138573.
Abstract:
Copper Zinc superoxide dismutases are avital antioxidant enzymes that catalyze the disproportionation of superoxide anion to oxygen(O2)and hydrogen peroxide(H2O2)to guard cells against superoxide toxicity. The cytosolic CuZnSOD gene was cloned from Eucalyptus grandis×E.ophylla(GenBank Accession Number: JX138573).The cDNA nucleotide sequence analysis revealed that an open reading frame contains 459 bp nucleotide coding for 152 residues(15.2 ku). The full-length gene is amplified to construct expression vetor pET-CuZnSOD. The Escherichia coli is induced by 1 mmol·L-1 IPTG in 28 ℃ for 4 hours and enzyme activity assay result shows that enzyme activity has increased 19.9% than the control in 28 ℃

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备注/Memo

备注/Memo:
收稿日期: 2015-01-05
通信作者: 赵艳玲(1978-),女,助理研究员,博士,主要从事植物分子生物学的研究.E-mail:zhaoyl@hqu.edu.cn.
基金项目: 国家自然科学基金资助项目(31300497)
更新日期/Last Update: 2015-11-20