[1]侯莹,成志云,王立强,等.RNAi表达载体在哺乳动物中的研究进展[J].华侨大学学报(自然科学版),2015,36(3):314-320.[doi:10.11830/ISSN.1000-5013.2015.03.0314]
 HOU Ying,CHENG Zhi-yun,WANG Li-qiang,et al.Research Progress of RNA Interference Expression Vector in Mammals[J].Journal of Huaqiao University(Natural Science),2015,36(3):314-320.[doi:10.11830/ISSN.1000-5013.2015.03.0314]
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RNAi表达载体在哺乳动物中的研究进展()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第36卷
期数:
2015年第3期
页码:
314-320
栏目:
出版日期:
2015-05-20

文章信息/Info

Title:
Research Progress of RNA Interference Expression Vector in Mammals
文章编号:
1000-5013(2015)03-0314-07
作者:
侯莹 成志云 王立强 许瑞安 唐明青
华侨大学 生物医学学院, 福建 泉州 362021
Author(s):
HOU Ying CHENG Zhi-yun WANG Li-qiang XU Rui-an TANG Ming-qing
School of Biomedical Sciences, Huaqiao University, Quanzhou 362021, China
关键词:
RNA干扰 表达载体 短发夹式RNA载体 微小RNA载体 哺乳动物
Keywords:
RNA interference expression vector small hairpin RNA vector microRNA vector mammals
分类号:
Q7
DOI:
10.11830/ISSN.1000-5013.2015.03.0314
文献标志码:
A
摘要:
对RNA干扰(RNAi)表达载体的非生物与生物递送系统及主流应用进行综述,明确其在基因调控及未来基因治疗领域的应用前景,并指出其在种属特异性及表达效率等方面存在的局限性.对RNAi表达载体的结构组成、发展沿革及体内作用机制进行归纳分析,提出基于结构组成和加工机制双重属性的分类方法.
Abstract:
This paper reviewed the non-biological and biological delivery systerms of RNA interference expression vector as well as the major applications, and cleared the application prospect in the area of gene regulation and gene therapy in the future, and also pointed out the limitation in the species-specificity and expression efficiency etc. This paper summarized and analyzed the structure composition and development evolution of the RNAi expression vector as well as the function mechanism in vivo, and proposed the classification based on the dual attributes of structure composition and processing mechanism.

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备注/Memo

备注/Memo:
收稿日期: 2015-01-05
通信作者: 唐明青(1982-),男,副教授,博士,主要从事腺相关病毒基因治疗的研究.E-mail:tangmingqing2222@163.com.
基金项目: 国家高技术研究发展计划(863计划)资助项目(2012AA020810); 国家自然科学基金资助项目(81271692); 福建省中青年教师教育科研项目(JA14020); 中央高校基本科研业务费专项资金资助项目(JB-JC1004); 华侨大学人才引进项目(14BS111)
更新日期/Last Update: 2015-05-20