[1]吴志超,陈国,苏鹏飞.羧基功能化超顺磁性纳米粒子吸附牛血清蛋白的特性[J].华侨大学学报(自然科学版),2015,36(3):303-308.[doi:10.11830/ISSN.1000-5013.2015.03.0303]
　WU Zhi-chao,CHEN Guo,SU Peng-fei.Adsorption Properties of Bovine Serum Albumin on Carboxyl-Functioned Superparamagnetic Nanoparticle[J].Journal of Huaqiao University(Natural Science),2015,36(3):303-308.[doi:10.11830/ISSN.1000-5013.2015.03.0303]
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羧基功能化超顺磁性纳米粒子吸附牛血清蛋白的特性()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第36卷
期数:
2015年第3期
页码:
303-308
栏目:
出版日期:
2015-05-20

文章信息/Info

Title:
Adsorption Properties of Bovine Serum Albumin on Carboxyl-Functioned Superparamagnetic Nanoparticle
文章编号:
1000-5013(2015)03-0303-06
作者:
吴志超;  陈国;  苏鹏飞
华侨大学 化工学院, 福建 厦门 361021
Author(s):
WU Zhi-chao;  CHEN Guo;  SU Peng-fei
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
羧基磁性纳米粒子;  牛血清蛋白;  吸附;  蛋白固定化;  动力学
Keywords:
carboxyl magnetic nanoparticles;  bovine serum albumin;  adsorption;  protein immobilization;  kinetics
分类号:
O647
DOI:
10.11830/ISSN.1000-5013.2015.03.0303
文献标志码:
A
摘要:
以牛血清白蛋白(BSA)为目标蛋白,考察时间、BSA质量浓度、pH值、温度等条件对磁性纳米粒子吸附BSA的影响,并对吸附有BSA的磁性纳米粒子的解吸附情况进行研究.结果表明:在25~45 ℃内,温度几乎不影响吸附过程;pH值为4.0~4.5达最大吸附量,该吸附过程满足准一级动力学方程和Freundlich等温吸附模型;Na2HPO4对其有较强的脱附作用,但粒子与BSA之间不是简单的物理相互作用,而是静电相互作用,导致其脱附水平依然较低,仅为20.06%.
Abstract:
Using bovine serum albumin(BSA)as the target protein, the effect of time, BSA concentration, pH, temperature on magnetic nanoparticles(MNP)of BSA was investigated, as well as the desorption of BSA from the MNP. The results showed that the temperature nearly had no effect on adsorption process when it was 25-45 ℃. It reached the max adsorption amount when pH was 4.0-4.5, and the adsorption process fitted the quasi first order kinetic equation and Freundlich isotherm adsorption model. Na2HPO4 had a strong effect on the desorption process, but the desorption rate was still low of only 20.06%. This is because it is not a simple physical interaction but the electrostatic interaction between the particle and BSA.

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备注/Memo

备注/Memo:
收稿日期: 2014-09-23
通信作者: 陈国(1978-),男,副教授,博士,主要从事生化反应工程和生化分离工程的研究.E-mail:chenguo@hqu.edu.cn.
基金项目: 国家自然科学基金资助项目(20906035); 华侨大学中青年教师科研提升资助计划项目(ZQN-PY109)
更新日期/Last Update: 2015-05-20