[1]金春英,林金清,王蔚.白斑综合症病毒对对虾Caspase基因的调控[J].华侨大学学报(自然科学版),2014,35(5):537-541.[doi:10.11830/ISSN.1000-5013.2014.05.0537]
　JIN Chun-ying,LIN Jin-qng,WANG Wei.Regulation of Prawn Caspase Gene by White Spot Syndromic Virus[J].Journal of Huaqiao University(Natural Science),2014,35(5):537-541.[doi:10.11830/ISSN.1000-5013.2014.05.0537]
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白斑综合症病毒对对虾Caspase基因的调控()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第35卷
期数:
2014年第5期
页码:
537-541
栏目:
出版日期:
2014-09-20

文章信息/Info

Title:
Regulation of Prawn Caspase Gene by White Spot Syndromic Virus
文章编号:
1000-5013(2014)05-0537-05
作者:
金春英1;  林金清2;  王蔚3
1. 华侨大学 化工学院, 福建 厦门 361021;2. 华侨大学 材料科学与工程学院, 福建 厦门 361021;3. 国家海洋局第三海洋研究所, 福建 厦门 361005
Author(s):
JIN Chun-ying1;  LIN Jin-qng2;  WANG Wei3
1. College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2. College of Material Science and Engineering, Huaqiao University, Xiamen 361021, China; 3. Third Institute of Oceanography, State Oceanic Administration(SOA), Xiamen 361005, C
关键词:
对虾白斑综合症病毒;  Caspase基因;  调控;  凋亡
Keywords:
white spot syndromic virus;  Caspase gene;  regulation;  apoptosis
分类号:
Q516
DOI:
10.11830/ISSN.1000-5013.2014.05.0537
文献标志码:
A
摘要:
研究病毒感染对白斑综合症病毒基因的录调控的影响,将Caspase调控区的DNA进行生物素标记,然后与链霉亲和素修饰的琼脂糖结合.通过下拉发现,Caspase基因的启动子序列与white spot syndromic virus(WSSV)病毒的两个蛋白Vp38和Vp41B有相互作用.通过荧光素酶报告基因发现,Vp38和Vp41B对Caspase启动子活性分别有抑制和激活作用.采用RNAi技术下调两个WSSV蛋白的表达后,研究发现:Vp38和Vp41B分别对对虾血细胞的凋亡水平有促进和抑制的调控作用.
Abstract:
The prawn Caspase gene was previously found to play important roles in protecting host from viral infection. In order to further delineate the transcriptional regulation of Caspase in response to viral infection, the Caspase promoter was biotin-labeled and bind to streptavidin-beads. Through pulldown assay the Caspase promoter was founded to interact with two white spot syndromic virus(WSSV)proteins Vp38 and Vp41B. Luciferase reporter assay revealed that Vp38 and Vp41B act as repressor and activator of Caspase transcription respectively. RNAi inhibition of Vp38 and Vp41B indicate the two WSSV proteins play roles on the regulation of prawn hymocyte apoptosis. These results provided insights into the immune mechanism of invertebrates, and will also be helpful for shrimp viral disease control.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期: 2013-12-28
通信作者: 金春英(1976-),女,实验师,主要从事免疫防治的研究.E-mail:jcying@hqu.edu.cn.
基金项目: 国家自然科学基金资助项目(31272684); 国家海洋局海洋生物遗传资源重点实验室开放研究基金项目(HY201102)
更新日期/Last Update: 2014-09-20