[1]葛慧华,张光亚,王士斌.氨基酸对类弹性蛋白多肽产量的影响[J].华侨大学学报(自然科学版),2013,34(5):542-546.[doi:10.11830/ISSN.1000-5013.2013.05.0542]
 GE Hui-hua,ZHANG Guang-ya,WANG Shi-bin.Influence of Amino Acids on the Production of Elastin-Like Polypeptides [KV8F-40][J].Journal of Huaqiao University(Natural Science),2013,34(5):542-546.[doi:10.11830/ISSN.1000-5013.2013.05.0542]
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氨基酸对类弹性蛋白多肽产量的影响()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第34卷
期数:
2013年第5期
页码:
542-546
栏目:
出版日期:
2013-09-20

文章信息/Info

Title:
Influence of Amino Acids on the Production of Elastin-Like Polypeptides [KV8F-40]
文章编号:
1000-5013(2013)05-0542-05
作者:
葛慧华 张光亚 王士斌
华侨大学 化工学院, 福建, 厦门 362021
Author(s):
GE Hui-hua ZHANG Guang-ya WANG Shi-bin
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
类弹性蛋白多肽 条件优化 氨基酸 响应面设计
Keywords:
elastin-like polypeptides medium optimization amino acids response surface method
分类号:
TQ936.16
DOI:
10.11830/ISSN.1000-5013.2013.05.0542
文献标志码:
A
摘要:
采用谷氨酰胺来确定氨基酸初始添加浓度,分析各种氨基酸对类弹性蛋白多肽[KV8F-40]产量的影响.结果表明:苏氨酸、精氨酸和半胱氨酸都能较大提高产量,其中以苏氨酸最为明显,产量可达126.9 mg·L-1,比对照增加54.0%.利用响应面设计对上述3种氨基酸进行3因素3水平实验,经优化后所得理论最大产量为100.3 mg·L-1.经实验验证,所得实验值为103.1 mg·L-1,与理论值吻合,但其产量低于单个氨基酸.交互作用分析表明:3种对产量有促进作用的氨基酸之间没有互作或者存在拮抗作用.
Abstract:
Using Gln to tentatively identify the initial concentration of each amino acid, we analyzed the influence of amino acids on the production of elastin-like polypeptides [KV8F-40]. The results showed Thr, Arg and Cys could improve the production at the concentration of 1.5 mmol·L-1. Among them, Thr was the best; it could improve the production from 82.4 mg·L-1 to 126.9 mg·L-1, with an improvement of 54%. And then, we designed the response surface analysis with three factors and three levels to optimize the cultivation medium of ELPs40. The experimental results fitted the theoretical result very well, which was 103.1 mg·L-1 and 100.3 mg·L-1, respectively. However, the production was less than that of single factor. By means of interaction analysis, we found Thr and Cys inhibit each other, while others didn’t have interaction.

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相似文献/References:

[1]王文研,张光亚.ELPs-PDOR融合基因表达条件的优化[J].华侨大学学报(自然科学版),2012,33(3):296.[doi:10.11830/ISSN.1000-5013.2012.03.0296]
 WANG Wen-yan,ZHANG Guang-ya.Optimization of Cultivation Conditions of ELPs-PDOR Fusion Gene[J].Journal of Huaqiao University(Natural Science),2012,33(5):296.[doi:10.11830/ISSN.1000-5013.2012.03.0296]

备注/Memo

备注/Memo:
收稿日期: 2012-09-28
通信作者: 王士斌(1965-),男,教授,主要从事生物医学材料的研究.E-mail:sbwang@hqu.edu.cn.
基金项目: 华侨大学高层次人才科研启动项目(10BS220)
更新日期/Last Update: 2013-09-20