[1]龙瑞敏,谢茂彬,王士斌.液体深层培养趋磁细菌AMB-1的生长及产磁能力[J].华侨大学学报(自然科学版),2013,34(4):415-421.[doi:10.11830/ISSN.1000-5013.2013.04.0415]
　LONG Rui-min,XIE Mao-bin,WANG Shi-bin.Study on AMB-1 Growth and Magnetism Generation Capacity by Submerged Liquid Cultivation[J].Journal of Huaqiao University(Natural Science),2013,34(4):415-421.[doi:10.11830/ISSN.1000-5013.2013.04.0415]
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液体深层培养趋磁细菌AMB-1的生长及产磁能力()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第34卷
期数:
2013年第4期
页码:
415-421
栏目:
出版日期:
2013-07-20

文章信息/Info

Title:
Study on AMB-1 Growth and Magnetism Generation Capacity by Submerged Liquid Cultivation
文章编号:
1000-5013(2013)04-0415-07
作者:
龙瑞敏1;  谢茂彬1;  王士斌1; 2
1. 华侨大学 化工学院, 福建 厦门 361021; 2. 华侨大学 生物材料与组织工程研究所, 福建 厦门 361021
Author(s):
LONG Rui-min1;  XIE Mao-bin1;  WANG Shi-bin1; 2
1. College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2. Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, China
关键词:
液体深层培养;  AMB-1;  生物量;  磁小体
Keywords:
submerged liquid cultivation;  AMB-1;  biomass;  magnetosome
分类号:
TQ920.6
DOI:
10.11830/ISSN.1000-5013.2013.04.0415
文献标志码:
A
摘要:
采用液体深层培养法培养趋磁细菌AMB-1,考察装瓶量、铁源种类及浓度、碳源种类及浓度、氮源种类及浓度、混合碳源比例共8个因素对AMB-1生长和产磁能力的影响.研究结果表明:AMB-1能够稳定生长并合成磁小体,但是碳源种类及氮源种类对AMB-1的生长影响较为明显;除琥珀酸外,AMB-1无法利用酒石酸、乙酸钠和柠檬酸作为单一碳源,而硝酸钠为最佳氮源.
Abstract:
AMB-1 was cultured by submerged liquid cultivation. Different parameters including bottling amount, iron source and concentration, carbon source and concentration, nitrogen source and concentration and the proportion of mixed carbon source were investigated on the effects of AMB-1 growth and magnetism generation capacity. Results indicated that AMB-1 could grow steadily and synthesize magnetosomes under all parameters, but carbon source and nitrogen source both had great influence on AMB-1 growth. AMB-1 couldn’t use tartaric acid, sodium acetate and citric acid as single carbon source except succinic acid, and NaNO3 was the optimal nitrogen source. These results provided referencable data for application and dissemination of magnetosomes production by AMB-1 under common experimental conditions.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期: 2012-12-03
通信作者: 龙瑞敏(1979-),女,实验员,主要从事材料的开发与应用的研究.E-mail:simplever@126.com.
基金项目: 国家自然科学基金资助项目(31000441); 华侨大学科研基金资助项目(10HZR10)
更新日期/Last Update: 2013-07-20