[1]张光亚.嗜盐蛋白高级结构对其稳定性的影响[J].华侨大学学报(自然科学版),2012,33(6):655-659.[doi:10.11830/ISSN.1000-5013.2012.06.0655]
　ZHNAG Guang-ya.Influence of Advanced Structures on Protein Halophilic Stability[J].Journal of Huaqiao University(Natural Science),2012,33(6):655-659.[doi:10.11830/ISSN.1000-5013.2012.06.0655]
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嗜盐蛋白高级结构对其稳定性的影响()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第33卷
期数:
2012年第6期
页码:
655-659
栏目:
出版日期:
2012-11-20

文章信息/Info

Title:
Influence of Advanced Structures on Protein Halophilic Stability
文章编号:
1000-5013(2012)06-0655-05
作者:
张光亚
华侨大学 化工学院, 福建 厦门 361021
Author(s):
ZHNAG Guang-ya
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
嗜盐蛋白;  非嗜盐蛋白;  二级结构;  可及性表面积;  溶剂可及性;  嗜盐机制
Keywords:
halophilic protein;  non-halophilic protein;  secondary structure;  accessible surface area;  solvent accessibility;  halophilic mechanism
分类号:
O517
DOI:
10.11830/ISSN.1000-5013.2012.06.0655
文献标志码:
A
摘要:
选择30对具有晶体结构的嗜盐和非嗜盐同源蛋白,通过计算氨基酸所处的结构状态,找出二者在统计学上具有显著差异的氨基酸.研究结果表明:在总的氨基酸组成上,嗜盐蛋白中天冬氨酸含量更多,而苯丙氨酸和赖氨酸含量显著较少.在不同二级结构区域,β-折叠中两种蛋白氨基酸组成差异最大;而在不同溶剂可及表面,两种蛋白在分别表面氨基酸组成差异最大,嗜盐蛋白表面酸性氨基酸显著较多,而碱性氨基酸则较少;48种高级结构结构参数中有4种存在显著差异,这4种结构参数都与可及性表面积有关.
Abstract:
To investigate the structural distribution responsible for protein halophilicity is of great significance for understanding the stability of halophilic protein and would help to develop a practical strategy for designing halophilic proteins. A systematic comparative analysis of 30 pairs of halophilic and non-halophilic proteins was reported. The residue structu-ral states based on secondary structure and solvent accessibility were considered for analyzing the structural patterns of single amino acids. The statistical test revealed that higher frequency in overall of Asp at the expense of Phe and Lys, higher frequency in exposed state of acid amino acids at the expense of alkaline amino acids. The β-sheet and the exposed surface were the regions showed great differences. Among the 48 structure parameters, there were four parameters which showed significant difference between the two kinds of proteins. Interestingly, the four parameters were all related with the accessible surface area.

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备注/Memo

备注/Memo:
收稿日期: 2012-02-05
通信作者: 张光亚(1975-),男,教授,主要从事酶学与生物信息学的研究.E-mail:zhgyghh@hqu.edu.cn.
基金项目: 国家自然科学基金资助项目(20806031); 福建省自然科学基金资助项目(2009J01030); 福建省高校新世纪优秀人才支持计划项目(07176C02)
更新日期/Last Update: 2012-11-20