[1]陈爱明,林毅.Ⅱ型抗癌晶体蛋白的芳香族氨基酸及其与抗肝癌活性间的关系[J].华侨大学学报(自然科学版),2012,33(4):408-411.[doi:10.11830/ISSN.1000-5013.2012.04.0408]
　CHEN Ai-ming,LIN Yi.Relationship Between Aromatic Amino Acids of Parasporin-2 and its Anti-Liver-Cancer Activity[J].Journal of Huaqiao University(Natural Science),2012,33(4):408-411.[doi:10.11830/ISSN.1000-5013.2012.04.0408]
点击复制

Ⅱ型抗癌晶体蛋白的芳香族氨基酸及其与抗肝癌活性间的关系()
分享到：

《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第33卷
期数:
2012年第4期
页码:
408-411
栏目:
出版日期:
2012-07-20

文章信息/Info

Title:
Relationship Between Aromatic Amino Acids of Parasporin-2 and its Anti-Liver-Cancer Activity
文章编号:
1000-5013(2012)04-0408-04
作者:
陈爱明;  林毅
华侨大学 化工学院, 福建 厦门 361021
Author(s):
CHEN Ai-ming;  LIN Yi
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
Ⅱ型抗癌晶体蛋白;  苏云金芽孢杆菌;  芳香族氨基酸;  分子对接;  甘油醛-3-磷酸脱氢酶
Keywords:
parasporin-2;  Bacillus thuringiensis;  aromatic amino acids;  molecular docking;  glyceraldehyde-3-phosphate dehydrogenase
分类号:
Q511.04;Q78
DOI:
10.11830/ISSN.1000-5013.2012.04.0408
文献标志码:
A
摘要:
利用氨基酸序列比对,蛋白质间相互作用位点预测和蛋白质与蛋白质对接,研究Ⅱ型抗癌晶体蛋白的氨基酸组成与其抗肝癌活性之间关系.结果表明:Ⅱ型抗癌晶体蛋白分子上位点49,51,52,55~60,194和205~212的氨基酸残基,特别是芳香族氨基酸在配体和受体蛋白质间的相互作用中起着重要作用;在Hex的蛋白质与蛋白质对接中,配体P11和P32与受体甘油醛-3-磷酸脱氢酶(GAPDH)的结合能量最低,表明P11和P32更容易与GAPDH结合.
Abstract:
In this study, amino sequences blast, protein-protein interaction sites prediction and protein-protein docking were employed to explore the relationship between amino acid composition of parasporin-2 and its anti-liver-cancer activity. The results showed that amino residues, especially the aromatic amino residues at sites 49, 51, 52, 55-60, 194 and 205-212 in parasporin-2 were found to play a key role in ligand-receptor interaction. Protein-protein docking on Hex illustrated that ligands P11 and P32 had lower total binding energy value than the others when glyceraldehyde-3-phosphate dehydrogenase(GAPDH)was the receptor, indicated that P11 and P32 were easier to bind to GAPDH.

参考文献/References:

[1] MIZUKI E,OHBA M,AKAO T,et al.Unique activity associated with non-insecticidal Bacillus thuringiensis parasporal inclusions:In vitro cell-killing action on human cancer cells[J].Journal of Applied Microbiology,1999,86(3):477-486.
[2] LEE D W,AKAO T,YAMASHITA S,et al.Noninsecticidal parasporal proteins of a Bacillus thuringiensis serovar shandongiensis isolate exhibit a preferential cytotoxicity against human leukemic T cells[J].Biochemical and Biophysical Research Communications,2000,272(1):218-223.
[3] ITO A,SASAQURI Y,KITADA S,et al.A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells[J].The Journal of Biological Chemistry,2004,279(20):21282-21286.
[4] MIZUKI E,PARK Y S,SAITOH H,et al.Parasporin, a human leukemic cell-recognizing parasporal protein of Bacillus thuringiensis[J].Clinical and Diagnostic Laboratory Immunology,2000,7(4):625-634.
[5] ABE Y,KITADA S,KUGE O,et al.Oligomerization of parasporin-2,a new crystal protein from non-insecticidal Bacillus thuringiensis, in lipid rafts[C]//6th Pacific Rim Conference on the Biotechnology of Bacillus thuringiensis and its Environmental Impact.Victoria B C:[s,n],2005:113-115.
[6] KITADA S,ABE Y,MAEDA T,et al.Parasporin-2 requires GPI-anchored proteins for the efficient cytocidal action to human hepatoma cells[J].Toxicology,2009,264(1/2):80-88.
[7] KRISHNAN K,KER J E A,MOHAMMED S M,et al.Identification of glyceraldehyde-3-phosphate dehydrogenase(GAPDH)as a binding protein for a 68 kDa Bacillus thuringiensis parasporal protein cytotoxic against leukaemic cells[J].Journal of Biomedical Science,2010,17(1):86.
[8] OKUMURA S,SAITOH H,ISHIKAWA T,et al.Mode of action of parasporin-4, a cytocidal protein from Bacillus thuringiensis[J].Biochimica et Biophysica Acta,2011,1808(6):1476-1482.
[9] AKIBA T,ABE Y,KITADA S,et al.Crystal structure of the parasporin-2 Bacillus thuringiensis toxin that recognizes cancer cells[J].Journal of Molecular Biology,2009,386(1):121-133.
[10] 岳俊杰,冯华,梁龙.蛋白质结构预测实验指南[M].北京:化学工业出版社,2010.

备注/Memo

备注/Memo:
收稿日期: 2011-11-22
通信作者: 林毅(1976-),男,教授,主要从事伴孢晶体蛋白的基因克隆与信息学的研究.E-mail:lyhxm@hqu.edu.cn.
基金项目: 教育部科学技术研究重点项目(211205); 福建省自然科学基金资助项目(2011J01221); 福建省高等学校新世纪优秀人才支持计划(2006年度)
更新日期/Last Update: 2012-07-20