[1]洪雪梅,林毅.新型杀虫晶体蛋白Cry7Ab4空间结构的计算机解析[J].华侨大学学报(自然科学版),2012,33(1):60-64.[doi:10.11830/ISSN.1000-5013.2012.01.0060]
 HONG Xue-mei,LIN Yi.Structural Analysis of a Novel Insecticidal Crystal Protein Cry7Ab4[J].Journal of Huaqiao University(Natural Science),2012,33(1):60-64.[doi:10.11830/ISSN.1000-5013.2012.01.0060]
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新型杀虫晶体蛋白Cry7Ab4空间结构的计算机解析()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第33卷
期数:
2012年第1期
页码:
60-64
栏目:
出版日期:
2012-01-20

文章信息/Info

Title:
Structural Analysis of a Novel Insecticidal Crystal Protein Cry7Ab4
文章编号:
1000-5013(2012)01-0060-05
作者:
洪雪梅林毅
华侨大学信息科学与工程学院; 华侨大学化工学院
Author(s):
HONG Xue-mei1 LIN Yi2
1.College of Information Science and Engineering, Huaqiao University, Xiamen 361021, China; 2.College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
苏云金芽孢杆菌 杀虫晶体蛋白 Cry7Ab4 空间结构 构效关系
Keywords:
Bacillus thuringiensis insecticidal crystal protein Cry7Ab4 spatial structure structure-activity relationship
分类号:
S482.3
DOI:
10.11830/ISSN.1000-5013.2012.01.0060
文献标志码:
A
摘要:
采用一级结构分析,二级结构预测及同源比对建模的方法,模拟出新型杀虫晶体蛋白Cry7Ab4的空间结构,并进行动力学优化.结合其杀大猿叶甲活性数据与蛋白结构比对分析,发现Cry7Ab4与Cry7Aa1的活性差异主要归因于α3,α4上疏水性氨基酸的不同.
Abstract:
The initial spatial structure of a novel insecticidal crystal protein Cry7Ab4 was constructed by primary structure analysis,secondary structure prediction and homologous alignment modeling,and then optimized by molecular mechanics method.In addition,the structural difference between Cry7Ab4 and Cry7Ab1 was compared by NCBI Blastp.It showed that the hydrophobic amino acids in α3,α4 were responsible for their difference of insecticidal activities against Colaphellus bowringi.

参考文献/References:

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[9] SCHWARTZ J L, JUTEAU M, GROCHULSKI P. Restriction of intramolecular movements within the CrylAa toxin molecule of Bacillus thuringiensis through disulfide bond engineering [J]. FEBS Letters, 1997, (2/3):397-402.doi:10.1016/S0014-5793(97)00626-1.
[10] YAOI K, NAKANISHI K, KADOTANI T. Bacillus thuringiensis CrylAa toxin-binding region of Bombyx mori aminopeptidase N [J]. FEBS Letters, 1999(3):221-224.doi:10.1016/S0014-5793(99)01626-9.
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相似文献/References:

[1]蔡福营,樊云,张光亚,等.杀虫晶体蛋白对小菜蛾杀虫活性的识别预测[J].华侨大学学报(自然科学版),2009,30(1):53.[doi:10.11830/ISSN.1000-5013.2009.01.0053]
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备注/Memo

备注/Memo:
教育部科学技术研究重点项目(211205); 福建省高等学校新世纪优秀人才支持计划项目(2006年度); 福建省自然科学基金资助项目(2011J01221); 植物病虫害生物学国家重点实验室开放基金资助项目(2007IP7)
更新日期/Last Update: 2014-03-23