[1]邓轶韬,李夏兰,陈宗香,等.阿魏酸酯酶产生菌的筛选及产酶条件的优化[J].华侨大学学报(自然科学版),2011,32(3):300-303.[doi:10.11830/ISSN.1000-5013.2011.03.0300]
　DENG Yi-tao,LI Xia-lan,CHEN Zong-xiang,et al.Screening of Feruloyl Esterases Producing Strain and Optimization of the Enzyme Production[J].Journal of Huaqiao University(Natural Science),2011,32(3):300-303.[doi:10.11830/ISSN.1000-5013.2011.03.0300]
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阿魏酸酯酶产生菌的筛选及产酶条件的优化()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第32卷
期数:
2011年第3期
页码:
300-303
栏目:
出版日期:
2011-05-20

文章信息/Info

Title:
Screening of Feruloyl Esterases Producing Strain and Optimization of the Enzyme Production
文章编号:
1000-5013(2011)03-0300-04
作者:
邓轶韬; 李夏兰; 陈宗香; 蔡婀娜
华侨大学化工学院
Author(s):
DENG Yi-tao;  LI Xia-lan;  CHEN Zong-xiang;  CAI E-na
College of Chemical Engineering, Huaqiao University, Quanzhou 362021, China
关键词:
阿魏酸;  阿魏酸酯酶;  曲霉;  筛选
Keywords:
ferulic acid;  feruloyl esterase;  Aspergillus sp.;  screening
分类号:
TQ925
DOI:
10.11830/ISSN.1000-5013.2011.03.0300
文献标志码:
A
摘要:
从土壤中筛选到1株阿魏酸酯酶活力较高的菌株,通过形态观察,初步鉴定其为曲霉.通过单因素轮换法,对其液态发酵的产酶条件进行优化.结果表明:在查氏培养基中,以质量体积比为6%的麦麸为碳源,质量体积比为5%的硝酸钠为氮源,在30℃下发酵培养6 d后,其酶活力最高可达16.33μkat·L-1.
Abstract:
A strain producing feruloyl esterases was obtained from the soil.It was preliminarily identified as Aspergillus sp.according to the morphological characteristics.Production of feruloyl estserses was optimized by single factor method.Results showed that the highest enzyme activity could reach 16.33 μkat·L-1 after 6 days at 30 ℃ in Czapek medium containing 6%(mass/volume) wheat bran and 5%(mass/volume) sodium nitrate.

参考文献/References:

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更新日期/Last Update: 2014-03-23