[1]李琳,薛秀玲,连小彬.加速溶剂萃取-高效液相色谱法测定五氯苯酚[J].华侨大学学报(自然科学版),2011,32(3):292-295.[doi:10.11830/ISSN.1000-5013.2011.03.0292]
　LI Lin,XUE Xiu-ling,LIAN Xiao-bin.Determination of Pentachlorophenol in Leather by Accelerated Solvent Extraction and HPLC[J].Journal of Huaqiao University(Natural Science),2011,32(3):292-295.[doi:10.11830/ISSN.1000-5013.2011.03.0292]
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加速溶剂萃取-高效液相色谱法测定五氯苯酚()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第32卷
期数:
2011年第3期
页码:
292-295
栏目:
出版日期:
2011-05-20

文章信息/Info

Title:
Determination of Pentachlorophenol in Leather by Accelerated Solvent Extraction and HPLC
文章编号:
1000-5013(2011)03-0292-04
作者:
李琳; 薛秀玲; 连小彬
华侨大学化工学院; 晋江出入境检验检疫局
Author(s):
LI Lin1;  XUE Xiu-ling1;  LIAN Xiao-bin2
1.College of Chemical Engineering, Huaqiao University, Quanzhou 362021, China; 2.Jinjiang Entry-Exit Inspection and Quarantine Bureau, Quanzhou 362200, China
关键词:
五氯苯酚;  加速溶剂萃取;  高效液相色谱法;  皮革制品;  正己烷
Keywords:
pentachlorophenol;  accelerated solvent extraction;  high-performance liquid chromatography;  leather;  N-hexane
分类号:
TS107; TS57
DOI:
10.11830/ISSN.1000-5013.2011.03.0292
文献标志码:
A
摘要:
建立加速溶剂萃取-高效液相色谱联用的方法,检测皮革中五氯苯酚的残余量.采用正己烷为溶剂,皮革样品经加速溶剂萃取、浓缩、过滤后,以乙腈和0.5%的乙酸水溶液为流动相,通过C18高效液相色谱柱,在二极管阵列检测器波长为214 nm处进行检测,以标准物质保留时间定性及外标法定量分析.结果表明:五氯苯酚在质量浓度为0.1~20.0 mg·L-1范围内具有良好的线性关系(r=0.999 8),平均回收率为96.0%~99.7%,相对标准偏差为0.74%~5.42%,检测限为0.02μg·g-1.
Abstract:
A method of accelerated solvent extraction and high performance liquid chromatography(HPLC) was developed for the determination of residue pentachlorophenol in leather.The leather samples were extracted using accelerated solvent extraction(ASE) with N-hexane,then enriched and filtrated.Pentachlorophenol(PCP) was eluted on a C18 column with acetonitrile and 0.5% acetic acid solution,which then were detected by diode array detection(DAD) at 214 nm.Qualitative and quantitative analysis was carried out by retention time of PCP and external standard method.The results show that the mass concentration of PCP was a linear range of 0.1~20 mg·g-1 with good correlation coefficients(r=0.999 8),the mean recoveries were 96.0%~99.7%,with the relative standard deviation were 0.74%~5.42%,and the detection limit of this method for PCP was 0.02 μg·g-1.

参考文献/References:

[1] 孙磊, 蒋新, 周健民. 环境样品中五氯苯酚分析方法的研究进展 [J]. 分析科学学报, 2004(6):642-646.doi:10.3969/j.issn.1006-6144.2004.06.026.
[2] ISO. ISO 17070-2006.Leather:Chemical tests:Determination of pentachlorophenol content [S]. ISO, 2006.
[3] 中华人民共和国进出口商品检验局. SN 0193.1-1993.出口皮革及皮革制品中五氯酚残留量检验方法 [S]. 北京, 1993.
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[7] SINGH S, CHANDRA R, PATEL D K. Investigation of the biotransformation of pentachlorophenol and pulp paper mill effluent decolorisation by the bacterial strains in a mixed culture [J]. Bioresource Technology, 2008, (13):5703-5709.doi:10.1016/j.biortech.2007.10.022.
[8] LIU Xi-tao, QUAN Xie, BO Long-li. Simultaneous pentachlorophenol decomposition and granular activated carbon regeneration assisted by microwave irradiation [J]. CARBO, 2004(2):415-422.doi:10.1016/j.carbon.2003.12.032.
[9] 刘静. 戴安公司ASE快速溶剂萃取技术:解决您化学实验样品前处理的最新技术 [J]. 检验检疫科学, 2003(2):58.doi:10.3969/j.issn.1674-5354.2003.02.024.

更新日期/Last Update: 2014-03-23