[1]陈碧强,林毅,明艳林,等.三种中草药对人肝癌细胞SMMC7721体外增殖抑制[J].华侨大学学报(自然科学版),2009,30(4):425-428.[doi:10.11830/ISSN.1000-5013.2009.04.0425]
　CHEN Bi-qiang,LIN Yi,MING Yan-lin,et al.Inhibitory Effect of Three Kinds of Chinese Herbs on Human Hepatoma Cell Line SMMC7721 in Vitro[J].Journal of Huaqiao University(Natural Science),2009,30(4):425-428.[doi:10.11830/ISSN.1000-5013.2009.04.0425]
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三种中草药对人肝癌细胞SMMC7721体外增殖抑制()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第30卷
期数:
2009年第4期
页码:
425-428
栏目:
出版日期:
2009-07-20

文章信息/Info

Title:
Inhibitory Effect of Three Kinds of Chinese Herbs on Human Hepatoma Cell Line SMMC7721 in Vitro
文章编号:
1000-5013(2009)04-0425-04
作者:
陈碧强; 林毅; 明艳林; 陈良华; 童庆宣
华侨大学化工学院; 厦门华侨亚热带植物引种园药用植物与植物药研究中心
Author(s):
CHEN Bi-qiang1;  LIN Yi1;  MING Yan-lin2;  CHEN Liang-hua2;  TONG Qing-xuan2
1.College of Chemical Engineering, Huaqiao University, Quanzhou 362021, China; 2.Research Center for Medical Plants and Plant Medicine, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, China
关键词:
中草药;  肝癌细胞SMMC7721;  四甲基偶氮唑盐比色法;  细胞凋亡;  体外增殖;  抑制
Keywords:
chinese herbs;  hepatoma cell line SMMC7721;  MTT assay;  cell apoptosis
分类号:
R735.7
DOI:
10.11830/ISSN.1000-5013.2009.04.0425
文献标志码:
A
摘要:
采用四甲基偶氮唑盐(MTT)比色法和细胞凋亡形态观察,初步探讨白英、毛麝香和使君子3种中草药的醇提物对肝癌细胞SMMC7721体外增殖的抑制效果.结果表明,白英醇提物对肝癌细胞SMMC7721有很强的抑制作用,当抑制率为50%时,样品的质量浓度(5ρ0)达到25.85 mg.L-1,且经50 mg.L-1作用72 h后,细胞逐渐变圆,体积缩小,有凋亡小体形成,呈典型的凋亡现象; 使君子、毛麝香的醇提物对细胞抑制作用较弱,在药物质量浓度达到100 mg.L-1时,对SMMC7721细胞的抑制率分别为43.88%,20.88%,二者的5ρ0均大于100 mg.L-1.
Abstract:
The anti-proliferative potential of ethanol extracts from Solanum lyratum,Adenosma glutinosum,and Quisqualis indica against hepatoma cell line SMMC7721 were evaluated in vitro by MTT assay and morphologigcal observation.Ethanol extract from Solanum lyratum demonstrated strong inhibitory effect.When the value of ρ50 was 25.85 mg·L-1,the inhibitory rate reached 50%.Apoptotic characteristics,including chromatin condensation,cell shrinkage,and the appearance of apoptotic bodies,were observed with 50 mg·L-1 for 72 h.The other two extracts from Adenosma glutinosum and Quisqualis indica had faint inhibitory effects,the inhibitory rate with 100 mg·L-1 were 43.88% and 20.88%,respectively.

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金资助项目(40601046); 厦门市科技创新基金重点项目(3502Z20062008)
更新日期/Last Update: 2014-03-23