[1]王耿,方柏山,张婷婷,等.一株碱性脱毛蛋白酶产生菌的筛选及系统发育分析[J].华侨大学学报(自然科学版),2009,30(3):297-301.[doi:10.11830/ISSN.1000-5013.2009.03.0297]
　WANG Geng,FANG Bai-shan,ZHANG Ting-ting,et al.Isolation and Phylogenetic Analysis of a Strain Producing Alkaline Protease for Dehairing[J].Journal of Huaqiao University(Natural Science),2009,30(3):297-301.[doi:10.11830/ISSN.1000-5013.2009.03.0297]
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一株碱性脱毛蛋白酶产生菌的筛选及系统发育分析()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第30卷
期数:
2009年第3期
页码:
297-301
栏目:
出版日期:
2009-05-20

文章信息/Info

Title:
Isolation and Phylogenetic Analysis of a Strain Producing Alkaline Protease for Dehairing
文章编号:
1000-5013(2009)03-0297-05
作者:
王耿; 方柏山; 张婷婷; 靳鸿蔚; 刘桂兰
华侨大学工业生物技术福建省高等学校重点实验室
Author(s):
WANG Geng;  FANG Bai-shan;  ZHANG Ting-ting;  JIN Hong-wei;  LIU Gui-lan
Key Laboratory of Industrial Biotechnology of Fujian Province University, Huaqiao University, Quanzhou 362021, China
关键词:
短小芽孢杆菌;  碱性蛋白酶;  脱毛;  16SrDNA;  系统发育
Keywords:
Bacillus pumilus;  alkaline protease;  dehairing;  16 SrDNA;  phylogenesis
分类号:
Q93
DOI:
10.11830/ISSN.1000-5013.2009.03.0297
文献标志码:
A
摘要:
经过采集土样,分离、筛选得到一株产碱性蛋白酶的菌株,编号为A607.通过摇瓶发酵,对发酵上清液进行酶活测定和脱毛实验,证实所产生的酶具有蛋白酶水解活性和良好的脱毛能力,其酶活达到263.39μkat.L-1、比活力为1 215.24μkat.g-1.16S rDNA序列分析表明,该菌株与Bacillus pumilus具有高达99%的相似性.用PHYLIP程序将该菌株与报道的相关碱性蛋白酶产生菌进行系统发育进化分析,发现菌株A607与Bacillus pumilus处于同一分支.
Abstract:
A strain named A607 which yields of alkaline protease for dehairing was isolated from soil.After shaker flask fermentation,it is found that the A607 is of proteolytic activity(1 215.24 μkat·g-1 protein; 263.39 μkat·L-1) and displayed good dehairing activity in the culture solution.Analysis of 16S rDNA sequence suggested that the A607 strain was the closest relative of Bacillus pumilus with 99% sequence identity.The phylogenetic analysis was performed by PHYLIP.The result showed that A607 had a high degree of similarity to Bacillus pumilus according to the phylogenetic tree.

参考文献/References:

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备注/Memo

备注/Memo:
福建省科技重大专项(2005HZ03-3); 国务院侨办科研基金资助项目(06QZR07)
更新日期/Last Update: 2014-03-23