[1]刘青,戴德哉.氯苄基四氢小檗碱对大鼠肥厚心肌中KATP通道表达的影响[J].华侨大学学报(自然科学版),2008,29(3):395-398.[doi:10.11830/ISSN.1000-5013.2008.03.0395]
　LIU Qing,DAI De-zai.The Expression of ATP-Sensitive Potassium(KATP) Channel in Infarct Produced Myocardial Remodeling of Rats[J].Journal of Huaqiao University(Natural Science),2008,29(3):395-398.[doi:10.11830/ISSN.1000-5013.2008.03.0395]
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氯苄基四氢小檗碱对大鼠肥厚心肌中KATP通道表达的影响()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第29卷
期数:
2008年第3期
页码:
395-398
栏目:
出版日期:
2008-07-20

文章信息/Info

Title:
The Expression of ATP-Sensitive Potassium(KATP) Channel in Infarct Produced Myocardial Remodeling of Rats
文章编号:
1000-5013(2008)03-0395-04
作者:
刘青; 戴德哉
华侨大学材料科学与工程学院; 中国药科大学药理研究室 福建泉州362021; 江苏南京210009
Author(s):
LIU Qing1;  DAI De-zai2
1.College of Material Science and Engineering, Huaqiao University, Quanzhou 362021, China; 2.Institute of Moleculovr Medicine, Pharmaceutical University, Nanjng 210009, China
关键词:
心肌肥厚;  心梗;  ATP敏感性钾离子通道;  反转录-多聚酶链式反应
Keywords:
remodeling myocardium;  infarct;  ATP-sensitive potassium channels;  reverse transcription-polymeric chain reaction
分类号:
R96
DOI:
10.11830/ISSN.1000-5013.2008.03.0395
文献标志码:
A
摘要:
观察心梗诱导的大鼠肥厚心肌中三磷酸腺苷(ATP)敏感性钾离子通道(KATP)的表达,并探讨其影响因素.将雄性大鼠随机分为5组,分别是假手术组,手术组,普萘洛尔组,氯苄基四氢小檗碱组及内皮素受体拮抗剂组.手术组及药物组的大鼠结扎冠脉左前降支,术后饲养10 d,药物组于术后第6天开始给药,共给药5d,由反转录-多聚酶链式反应(RT-PCR)方法确定KATP通道的mRNA表达.实验结果表明,心梗诱导的大鼠肥大心肌中的KATP通道的mRNA表达明显上调,经药物干预后有不同程度的降低.心梗诱导的大鼠肥厚心肌中的KATP通道的mRNA的表达上调,其表达上调除与β受体有关外,Ca2+离子可能参与对KATP通道表达的影响.
Abstract:
Aim to observe the expression of ATP-sensitive potassium(KATP) channel in infarct produced myocardial remodeling and the factors were studied.Methods: male sprague-dawley rats randomly divided into five groups,sham-operated,operated and three treatment groups including propranolol,p-chlorobenzyltetrahydroberberine and endothelin receptor antagonist.Myocardial infarction was induced by the left anterior descending coronary artery was ligated with a silk suture.Sham-operated controls were treated likewise,except that the suture around the coronary artery was not closed.From 5 d the treatment groups were administered drugs after coronary artery ligation.All animals were killed on 10 d after ligation.The expression of mRNA for KATP channels was studied in rat hypertrophic myocardium using reverse transcription-polymeric chain reaction(RT-PCR).Results: The expression of KATP channel in infarct produced myocardial remodeling displayed up-regulted.After treatment the expression of KATP channel shown down-regulated in different degree.Conclusion: The expression of KATP channel in infarct produced myocardial remodeling displayed up-regulated.The factors involved in Ca2+ except β receptor,the effect of Ca2+ on the expression of KATP in infarct produced myocardial need study more.

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金重点资助项目(30230170); 华侨大学科研启动基金资助项目(05BS301)
更新日期/Last Update: 2014-03-23