[1]靳鸿蔚,刘桂兰,王耿,等.一株胶原酶产生菌的筛选及系统发育分析[J].华侨大学学报(自然科学版),2008,29(3):387-390.[doi:10.11830/ISSN.1000-5013.2008.03.0387]
 JIN Hong-wei,LIU Gui-lan,WANG Geng,et al.Isolation,Identification and Phylogenetic Analysis of a Collagenase Strain from Bacillus megaterium[J].Journal of Huaqiao University(Natural Science),2008,29(3):387-390.[doi:10.11830/ISSN.1000-5013.2008.03.0387]
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一株胶原酶产生菌的筛选及系统发育分析()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第29卷
期数:
2008年第3期
页码:
387-390
栏目:
出版日期:
2008-07-20

文章信息/Info

Title:
Isolation,Identification and Phylogenetic Analysis of a Collagenase Strain from Bacillus megaterium
文章编号:
1000-5013(2008)03-0387-04
作者:
靳鸿蔚刘桂兰王耿方柏山
华侨大学工业生物技术福建省高等学校重点实验室; 华侨大学工业生物技术福建省高等学校重点实验室 福建泉州362021; 福建泉州362021
Author(s):
JIN Hong-wei LIU Gui-lan WANG Geng FANG Bai-shan
Laboratory of Industrial Biotechnology of Fujian Province University, Huaqiao University, Quanzhou 362021, China
关键词:
巨大芽孢杆菌 胶原酶 16SrDNA序列 系统发育
Keywords:
collagenase Bacillus megaterium 16S rDNA sequence phylogenetic analysis
分类号:
Q93
DOI:
10.11830/ISSN.1000-5013.2008.03.0387
文献标志码:
A
摘要:
从所采集土样、水样中分离筛选到呈革兰氏染色阳性,柱状产芽孢编号为LY-t1的菌株.通过对此菌进行牛皮消化实验,并以Ⅰ型胶原为底物,测定该菌发酵培养液中胶原酶活性,确认此菌具有胶原酶活性.16S rDNA序列分析表明,分离菌株与Bacillus megaterium具有98%相似性.最后,用PHYLIP程序将该菌株与报道的相关胶原酶产生菌进行系统发育进化分析.
Abstract:
A collagenase strain named LY-t1 was isolated from soil and waste water samples of leather factory.Collagenolytic activity was detected using collagen Ⅰ as the substrate.The phylogentic analysis based on 16S rDNA suggested strain LY-t1 was the closest relative of Bacillus megaterium with 98% sequence identity.The phylogenetic analysis was performed by PHYLIP.This paper first reports Bacillus megaterium strain LY-t1 is a collagenase strain.

参考文献/References:

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备注/Memo

备注/Memo:
福建省重大科技专项(2005HZ03-3); 国务院侨务科研基金资助项目(06QZR07)
更新日期/Last Update: 2014-03-23