[1]刘慧杰,田蕴,熊小京,等.未培养微生物研究的两种新技术及其寻找功能基因的应用[J].华侨大学学报(自然科学版),2008,29(2):177-183.[doi:10.11830/ISSN.1000-5013.2008.02.0177]
　LIU Hui-jie,TIAN Yun,XIONG Xiao-jing,et al.Two Novel Methods for Uncultured Microorganisms Study and Their Application in Searching Functional Genes[J].Journal of Huaqiao University(Natural Science),2008,29(2):177-183.[doi:10.11830/ISSN.1000-5013.2008.02.0177]
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未培养微生物研究的两种新技术及其寻找功能基因的应用()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第29卷
期数:
2008年第2期
页码:
177-183
栏目:
出版日期:
2008-04-20

文章信息/Info

Title:
Two Novel Methods for Uncultured Microorganisms Study and Their Application in Searching Functional Genes
文章编号:
1000-5013(2008)02-0177-07
作者:
刘慧杰; 田蕴; 熊小京; 郑天凌
厦门大学生命科学学院; 厦门大学海洋与环境学院; 厦门大学生命科学学院 福建厦门361005
Author(s):
LIU Hui-jie1;  TIAN Yun1;  XIONG Xiao-jing2;  ZHENG Tian-ling1
1.School of Life Sciences, Xiamen University;
关键词:
未培养微生物;  宏基因组;  稳定同位素;  功能基因;  微生物生态
Keywords:
uncultured microorganism;  metagenome;  stable isotope probing;  functional genes;  microbial ecology
分类号:
Q93
DOI:
10.11830/ISSN.1000-5013.2008.02.0177
文献标志码:
A
摘要:
在微生物筛选过程中,99%的未培养微生物是用传统的方法无法分离的,这极大地限制了微生物生态领域中的多样性和利用生物资源等方面的研究.采用新技术成为研究未培养微生物的技术瓶颈.综述应用于未培养微生物的两种新技术——宏基因组和稳定同位素,探讨这两种新技术在寻找功能基因中的应用,特别是在生态环境中寻找功能微生物.探讨降解微生物及其功能基因对污染的生态环境进行的生物修复,为进一步研究微生物生态多样性及其功能基因的开发应用打下基础.
Abstract:
Based on the strategy to explore microbial resource from natural environment and the fact that the majority(about 99%) of microbes in diverse environments are unculturable till now by using traditional microbiological methods,scientists try hardrounding the bottleneck to set up novel techniques to culture and study those uncultured microorganisms.In this paper,two novel methods concerning metagenomics and stable isotope probing for above-mentioned study were introduced and the application of searching functional genes and functional microbes in ecological environment was also discussed.In addition,the suggestion of bioremediation of polluted environment with degrading microbes and their functional gene was proposed.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目(40576054,40476047); 福建省自然科学基金资助项目(D0610021,2005YZ1023)
更新日期/Last Update: 2014-03-23