[1]余晓芬,刘英丽,程珊影,等.麦糟水解液中单糖及脂肪酸的HPLC分析[J].华侨大学学报(自然科学版),2007,28(4):403-405.[doi:10.3969/j.issn.1000-5013.2007.04.018]
　YU Xiao-fen,LIU Ying-li,CHENG Shan-ying,et al.Analysis of Monosaccharides and Fatty Acid in the Liquors of Acid Hydrolysis on Brewery′s Spent Grain by HPLC[J].Journal of Huaqiao University(Natural Science),2007,28(4):403-405.[doi:10.3969/j.issn.1000-5013.2007.04.018]
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麦糟水解液中单糖及脂肪酸的HPLC分析()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第28卷
期数:
2007年第4期
页码:
403-405
栏目:
出版日期:
2007-10-20

文章信息/Info

Title:
Analysis of Monosaccharides and Fatty Acid in the Liquors of Acid Hydrolysis on Brewery′s Spent Grain by HPLC
文章编号:
1000-5013(2007)04-0403-03
作者:
余晓芬; 刘英丽; 程珊影; 李夏兰; 方柏山
华侨大学工业生物技术福建省高等学校重点实验室; 华侨大学工业生物技术福建省高等学校重点实验室 福建泉州362021; 福建泉州362021
Author(s):
YU Xiao-fen;  LIU Ying-li;  CHENG Shan-ying;  LI Xia-lan;  FANG Bai-shan
Laboratory of Industrial Biotechnology of Fujian Province University, Huaqiao University, Quanzhou 362021, China
关键词:
麦糟;  高效液相色谱;  单糖;  脂肪酸;  示差折光检测器
Keywords:
brewery′s spent grain;  HPLC;  monosaccharide;  fatty acid;  differential refractive index detector
分类号:
TQ920
DOI:
10.3969/j.issn.1000-5013.2007.04.018
文献标志码:
A
摘要:
建立高效液相色谱(HPLC)测定麦糟水解液中的单糖及脂肪酸的分析方法,检测条件:色谱柱为Aminex HPX-87H柱,柱温为50℃,流动相为5 mmol.L-1的硫酸,流速为0.4 mL.min-1,检测器为示差折光检测器.在此条件下,水解液中的单糖及脂肪酸的峰面积与浓度线性关系良好,相关系数R2在0.999 91~0.999 98之间,回收率为97.3%~101.5%.通过对样品的分析表明,麦糟水解液中不含有葡萄糖、甲酸及乙酰丙酸,木糖和阿拉伯糖的质量浓度比较高,乙酸的质量浓度比较低,单糖及脂肪酸的混合液得到很好的分离,分离过程在23 min内完成.
Abstract:
A method for analysis of monosaccharides and fatty acid in the liquors of acid hydrolysis on Brewery′s spent grain by high performance liquid chromatography(HPLC) is described.The operating conditions were Aminex HPX-87H column at 50 ℃ and differential refractive index detector,the mobile phase was 5 mmol·L-1 H2SO4 and the flow-rate was 0.4 mL·min-1.The monosaccharides and fatty acid in the liquors was measured in the anterior conditions.The correlation coefficient was 0.999 91~0.999 98 and the corresponding average recoveries were 97.3%~101.5%.Glucose,formic acid and levulinic acid were not detected in the liquors of acid hydrolysis and the concentration of xylose and arabinose was high,while the acetic acid was low.The mixture of monosaccharides and fatty acid was efficiently separated and the whole process of separation was finished within 23 minutes.

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金资助项目(20276026,20446004); 福建省科技计划重点项目(2003I020)
更新日期/Last Update: 2014-03-23