[1]贺淹才,陈路,周文刚.丝裂霉素诱变黑曲霉产植酸酶过程模型化[J].华侨大学学报(自然科学版),2006,27(2):189-192.[doi:10.3969/j.issn.1000-5013.2006.02.021]
　He Yancai,Chen Lu,Zhou Wengang.Modeling and Parameter Estimation on Strain Improvement in Phytase Producing Aspergillus niger by Mitomycin C Mutagenesis[J].Journal of Huaqiao University(Natural Science),2006,27(2):189-192.[doi:10.3969/j.issn.1000-5013.2006.02.021]
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丝裂霉素诱变黑曲霉产植酸酶过程模型化()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第27卷
期数:
2006年第2期
页码:
189-192
栏目:
出版日期:
2006-04-20

文章信息/Info

Title:
Modeling and Parameter Estimation on Strain Improvement in Phytase Producing Aspergillus niger by Mitomycin C Mutagenesis
文章编号:
1000-5013(2006)02-0189-04
作者:
贺淹才; 陈路; 周文刚
华侨大学材料科学与工程学院; 华侨大学材料科学与工程学院 福建 泉州 362021; 福建 泉州 362021
Author(s):
He Yancai;  Chen Lu;  Zhou Wengang
College of Material Science and Engineering, Huaqiao University, 362021, Quanzhou, China
关键词:
植酸酶;  黑曲霉;  丝裂霉素C;  诱变;  模型化;  参数估计
Keywords:
phytase;  aspergillus niger;  mitomycin C;  mutagenesis;  modeling;  parameter estimation
分类号:
Q933
DOI:
10.3969/j.issn.1000-5013.2006.02.021
文献标志码:
A
摘要:
用均匀设计分析方案,进行丝裂霉素C对黑曲霉化学诱变菌丝体和原生质体诱导产植酸酶选育,并以诱变时间(X1)和丝裂霉素C的浓度(X2)建立回归方程.实验筛选出具有4株较高酶活的菌株,诱变菌丝体得到2株酶活是始发菌株183.89%和147.55%; 诱变原生质体得到2株酶活是始发菌株395.52%和381. 98%.由响应面模型初步判断,在丝裂霉素C浓度为5~30 mg·L-1和诱变时间45~60 min时,丝裂霉素C 对黑曲霉诱变原生质体选育产植酸酶菌株的效果比对菌丝体诱变效果好.用均匀设计对丝裂霉素C诱变菌丝体和原生质体的效果进行回归分析,所得模型调整后的相关系数分别为89.02%和99.13%,模型拟合程度好,实验误差小,可以用此模型较好的对丝裂霉素C的黑曲霉菌丝体诱变进行分析和预测.
Abstract:
Uniform design was employed to optimize the process of mitomycin C mutation for strain improvement in phytase producing A. niger on the hyphae or the protoplast with the treating time (X1) and mitomycin C concentration (X2) as two factor regression equation. Four strains of higher phytase producing A. niger were obtained in the chemistry mutagenesis, their relative phytase activities were 183. 89% and 147. 55% of the originals from A. niger hyphae; and 395. 52%,381. 98% of the original from A. niger protoplasts, respectively. Their regression response surface model of the u-niform design show that mitomycin C mutation on A. niger protoplasts for strain improvement of phytase producing ability was better than that of on A. niger hyphae when mitomycin C concentration was 5~30 mg · L-1 and treating time was 45~60 min. Using the uniform design for the process of strain improvement by mitomycin C mutation on A. niger hyphae or protoplasts and using the experiment result analysis by regression, obtain a model and adjust the model parameter, the relative coefficients were 89.1% and 99.13%, the fit was good and experiment error was small, the model could be used in analysis and prediction for mutation strain improvement.

参考文献/References:

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备注/Memo

备注/Memo:
福建省自然科学基金资助项目(C040100011)
更新日期/Last Update: 2014-03-23