[1]肖美添,黄雅燕,郭养浩.固定化细胞海藻酸钙凝胶内的扩散特性[J].华侨大学学报(自然科学版),2005,26(4):408-411.[doi:10.3969/j.issn.1000-5013.2005.04.020]
　Xiao Meitian,Huang Yayan,Guo Yanghao.Diffusion Peculiarity of Substrates in Alginate Gel with Immobilized Cells[J].Journal of Huaqiao University(Natural Science),2005,26(4):408-411.[doi:10.3969/j.issn.1000-5013.2005.04.020]
点击复制

固定化细胞海藻酸钙凝胶内的扩散特性()
分享到：

《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第26卷
期数:
2005年第4期
页码:
408-411
栏目:
出版日期:
2005-10-20

文章信息/Info

Title:
Diffusion Peculiarity of Substrates in Alginate Gel with Immobilized Cells
文章编号:
1000-5013(2005)04-0408-04
作者:
肖美添; 黄雅燕; 郭养浩
华侨大学材料科学与工程学院; 福州大学药物生物技术与工程研究所 福建泉州362021; 福建泉州362021; 福建福州350002
Author(s):
Xiao Meitian1;  Huang Yayan1;  Guo Yanghao2
1.College of Material Science and Engineering, Huaqiao University, 362021, Quanzhou, China; 2.Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, 350001, Fuzhou, China
关键词:
固定化酵母细胞;  扩散特性;  有效扩散系数;  海藻酸钙
Keywords:
immobilization;  yeast cells;  diffusion peculiarity;  effective diffusivity;  alginate calcium
分类号:
O648
DOI:
10.3969/j.issn.1000-5013.2005.04.020
文献标志码:
A
摘要:
采用浸入法,测定葡萄糖、苯乙酮酸和扁桃酸等3种物质在固定化酵母细胞海藻酸钙凝胶内的有效扩散系数.实验结果表明,相同条件下,葡萄糖的有效扩散系数De随着海藻酸钠浓度的提高而下降,优化的海藻酸钠质量分数为0.02.葡萄糖的有效扩散系数大于扁桃酸和苯乙酮酸.初始包埋量为54.4 g.L-1的固定化酵母细胞经增殖8 h后,扁桃酸在胶珠内的有效扩散系数为0.36×10-4cm2.min-1,明显小于未增殖或未包埋细胞.
Abstract:
By immersion method,the authors meausured the effective diffusivity of glucose,phenyelglyoxylie phenylglyoxylie acid and mandelic acid within alginate calcium gel with immobilized yeast cells.Experimental results showed that,under the same conditions,although effective diffusivity of glucose decreased with the increase of alginate concentration,there existed an optimized concentration,0.02 by weight fraction.The effective diffusivity of glucose was higher than that of the other two substrates.The effective diffusivity of mandelic acid with initial entrapped immobilized yeast cells of 54.4 g·L-1 and passing through 8 hour incubation,attained 0.36×10-4 cm2·min-1,which was less remarkably than that of mandelic acid without incubation or without entrapped cells.

备注/Memo

备注/Memo:
福建省科技计划重点基金资助项目(2003H023); 福建省自然科学基金资助项目(E0310019)
更新日期/Last Update: 2014-03-23