[1]胡纯铿,白凤武,安利佳.自絮凝酵母细胞膜脂肪酸影响乙醇激活ATP酶[J].华侨大学学报(自然科学版),2005,26(3):251-254.[doi:10.3969/j.issn.1000-5013.2005.03.007]
　Hu Chunkeng,Bai Fengwu,An Lijia.Fatty Acid Composition of Plasma Membrane Affects in Vivo Ethanol Activation of ATPase in a Self-Flocculating Yeast[J].Journal of Huaqiao University(Natural Science),2005,26(3):251-254.[doi:10.3969/j.issn.1000-5013.2005.03.007]
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自絮凝酵母细胞膜脂肪酸影响乙醇激活ATP酶()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第26卷
期数:
2005年第3期
页码:
251-254
栏目:
出版日期:
2005-07-20

文章信息/Info

Title:
Fatty Acid Composition of Plasma Membrane Affects in Vivo Ethanol Activation of ATPase in a Self-Flocculating Yeast
文章编号:
1000-5013(2005)03-0251-04
作者:
胡纯铿; 白凤武; 安利佳
华侨大学材料科学与工程学院; 大连理工大学环境与生命学院; 大连理工大学环境与生命学院 福建泉州362011; 辽宁大连116024; 辽宁大连116024
Author(s):
Hu Chunkeng1;  Bai Fengwu2;  An Lijia2
1.College of Material Science and Engineering, Huaqiao University, 362021, Quanzhou, China; 2.School of Environmental and Biological Science and Technology, Dalian University of Technology, 116024, Dalian, China
关键词:
耐乙醇;  质膜ATP酶;  磷脂脂肪酸;  自絮凝酵母;  米氏常数
Keywords:
ethanol tolerance;  plasma membrane ATPase;  phospholipid fatty acid;  self-flocculating yeast;  K _m
分类号:
Q946
DOI:
10.3969/j.issn.1000-5013.2005.03.007
文献标志码:
A
摘要:
首次揭示酵母菌细胞膜磷脂脂肪酸组成对影响质膜ATP酶对乙醇刺激的响应.实验结果表明,细胞膜磷脂脂肪酸组成特点,对生长于未添加乙醇条件下的自絮凝酵母的质膜ATP酶活性没有影响,但却明显影响生长于添加乙醇(体积分数为0.01~0.10)的菌体质膜ATP酶对乙醇激活的敏感性.预培养于添加0.6mmol·L-1棕榈酸、亚油酸或亚麻酸条件下,菌体质膜ATP酶的最大激活水平分别为各自酶的基态水平(未激活)的3.6,1.5和1.2倍,而对照组(预培养于未添加脂肪酸条件下的菌体)的相应值为2.3倍.酶激活后,米氏常数Km、最适pH和对质膜ATP酶特异性抑制剂钒酸钠的敏感性等性质不变,但最大反应速度Vmax明显增加.实验结果还表明,细胞膜磷脂脂肪酸组成特点对提高菌体的耐乙醇能力越有利,其质膜ATP酶被乙醇激活的幅度越大,说明菌体耐乙醇能力的提高,与其质膜ATP酶对乙醇激活的敏感性的增加密切相关.
Abstract:
This work reveals for the first time that fatty acid composition of phospholipids in plasma membranes of yeast significantly affects the response of plasma membrane ATPase to ethanol stimuli. As shown experimentally, the characteristics of phospholipid fatty acid composition of plasma membranes significantly affected the sensitivities of plasma membrane ATPases to in vivo ethanol activation for a self-flocculating yeast growing in the presence of ethanol(volume fraction is 0.01~0.10), although they had no effect on the activities of the enzymes of the cells growing in the absence of ethanol. The maximal levels of activated plasma membrane ATPases for the cells culturing in advance in the presence of 0.6 mmol·L -1 palmitic, linoleic or linolenic acid were 3.6, 1.5 or 1.2-fold higher than the basal(inactivated) levels respectively, while 2.3-fold was the corresponding value for the control(cells culturing in advance in the absence of fatty acid). After enzyme activation, the K _m values, the optimal pH values and the sensitivities of plasma membrane ATPases to specific inhibitor sodium orthovanadate kept constant but V _ max increased significantly. As also shown experimentally, the more advantageous for the characteristics of phospholipid fatty acid composition of plasma membranes to enhance ethanol tolerance of yeast, the greater in vivo ethanol activation of plasma membrane ATPases. This illustrates a close correlation between the enhancement in ethanol tolerance of yeast and the increase in sensitivities of plasma membrane ATPase to in vivo ethanol activation.

参考文献/References:

[1] Serrano R, Kielland-Brandt M C, Fink G R. Yeast plasma membrane ATPase is essential for growth and has homology with (Na++K+), K+ and Ca2+-ATPase [J]. NATURE, 1986, (319):689-693.
[2] Cartwright C P, Veazey F J, Rose A H. Effect of ethanol on activity of the plasma membrane ATPase in, and accumulation of glycine by Saccharomyces cerevisiae [J]. Journal of General Microbiology, 1987.857-865.
[3] Rosa M F, Sa-Correia I. In vivo activation by ethanol of plasma membrane ATPase of Saccharomyces cerevisiae [J]. Applied and Environmental Microbiology, 1991.830-835.
[4] Coote P J, Jones M V, Seymour I J. Activity of the plasma H+-ATPase is a key physiological determinant of thermotolerance in Saccharomyces cerevisiae [J]. Microbiology, 1994.1881-1890.doi:10.1099/13500872-140-8-1881.
[5] Sigler K, Hoffer M. Mechanisms of acid extrusion in yeasts [J]. Biochimica et Biophysica Acta, 1991.375-391.doi:10.1016/0304-4157(91)90003-F.
[6] Hu Chunkeng, Bai Fengwu, An Lijia. Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg2+ via reduction in plasma membrane permeability [J]. Biotechnology Letters, 2003, (25):1191-1194.
[7] Dickinson D P, Isenberg I. Preparation of spheroplasts of Schizosaccharomyces pombe [J]. Journal of General Microbiology, 1982.651-654.
[8] Schibeci A, Rattray J B M, Kidby D K. Isolation and identification of yeast plasma membrane [J]. Biochimica et Biophysica Acta, 1973.15-25.doi:10.1016/0005-2736(73)90250-2.
[9] Serrano R. In vivo glucose activation of the yeast plasma membrane ATPase [J]. FEBS Letters, 1983.11-14.

备注/Memo

备注/Memo:
国家863高科技研究发展计划基金资助项目(2002AA647060)
更新日期/Last Update: 2014-03-23