[1]张光亚,陈迪,方柏山.1,3-丙二醇脱氢酶三级结构的模建[J].华侨大学学报(自然科学版),2004,25(4):426-429.[doi:10.3969/j.issn.1000-5013.2004.04.021]
　Zhang Guangya,Chen Di,Fang Baishan.Modeling the Tertiary Structure of 1,3-Propandiol Dehydrogenase[J].Journal of Huaqiao University(Natural Science),2004,25(4):426-429.[doi:10.3969/j.issn.1000-5013.2004.04.021]
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1,3-丙二醇脱氢酶三级结构的模建()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第25卷
期数:
2004年第4期
页码:
426-429
栏目:
出版日期:
2004-10-20

文章信息/Info

Title:
Modeling the Tertiary Structure of 1,3-Propandiol Dehydrogenase
文章编号:
1000-5013(2004)04-0426-04
作者:
张光亚; 陈迪; 方柏山
华侨大学材料科学与工程学院; 华侨大学材料科学与工程学院 福建泉州362021; 福建泉州362021; 福建泉州362021
Author(s):
Zhang Guangya;  Chen Di;  Fang Baishan
College of Mater. Sci. & Eng., Huaqiao Univ., 362021, Quanzhou, China
关键词:
1; 3-丙二醇脱氢酶;  三级结构;  同源建模法;  穿线法
Keywords:
1;  3-propanediol dehydrogenase;  tertiary structure;  homologous modeling;  threading
分类号:
TQ929
DOI:
10.3969/j.issn.1000-5013.2004.04.021
文献标志码:
A
摘要:
利用生物大分子数据库及生物信息学技术,对来源于Klebsiellapneumoniae的 1,3 丙二醇脱氢酶分子进行二级结构预测、多序列联配 .实验发现,其具有较保守的铁离子结合位点,而未发现常见的NAD(H)结合位点 .在此基础上,利用同源建模法和穿线法模建该酶的三级结构,进一步发现铁离子结合位点在空间上形成一口袋状结构 .探讨对其作用机制,同时发现该序列中存在 2个含铁元素的乙醇脱氢酶信号 .
Abstract:
Based on the knowledge of bioinformatics, secondary structure prediction and muti-sequencing aligment of 1,3-propanediol dehydrogenase from Klebsiella pneumoniae were performed with the biological macromolecule databases and softwares. The results showed that it contains a fairly conserved iron-binding motif instead of the commonly seen NAD(H)-binding motif. On this basis, homologous modeling and threading were used to model the tertiary structure. The results of homology modeling indicated that the conserved iron-binding motif formed a cleft where the Fe 2+ may be bounded. Two iron-containing alcohol dehydrogenases signatures were found in it.

参考文献/References:

[1] 修志龙. 微生物发酵法生产1, 3-丙二醇的研究进展 [J]. 微生物学通报, 2000(4):300-302.doi:10.3969/j.issn.0253-2654.2000.04.020.
[2] Barbirato F, Himmi E H, Conte T. 1, 3-propanediol production by fermentation: An interesting wayto valorize glycerin from the ester and ethanol industries [J]. Industrial Crops and Products, 1998(7):281-289.doi:10.1016/S0926-6690(97)00059-9.
[3] Frank A S, Betsy L L, Douglas C C. Construction and characterization of a 1, 3-propanediol operon [J]. Applied and Environmental Microbiology, 1998(1):98-105.
[4] Rost B. PHD:Predicting one-dimensional protein structure by profile based neural networks [J]. Methods in Enzymology, 1996, (266):525-539.
[5] 张成岗, 贺福初. 生物信息学方法与实践 [M]. 北京:科学出版社, 2002.140-141.
[6] Daniel R, Boenigk R, Gottschalk G. Purification of 1, 3-propanediol dehydrogenase from Citrobacter freundii and cloning, sequencing, and overexpression of the corresponding gene in Escherichia coli [J]. Journal of Bacteriology, 1995(8):2151-2156.
[7] Hassiba M, Regis M. Purification and characterization of the 1, 3-peopanediol dehydrogenase of Clostridium butyricum E5 [J]. Enzyme and Microbial Technology, 2000, (6):399-405.doi:10.1016/S0141-0229(00)00219-2.
[8] Talarico T L, Axelsson L T, Novotny L. Utilization of glycerol as a hydrogen acceptor by Lactobacillus reuteri: Purification of 1, 3-propanediol: NAD oxidoreductase [J]. Applied and Environmental Microbiology, 1990.943-948.
[9] Sergey N R, Jacky B, Sveta S. Glycerol dehydrogenase: Structure, specificity, and mechanism of a family III polyol dehydrogenase [J]. Structure, 2001(9):789-802.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目(20276026); 福建省自然科学重点基金资助项目(D0120002)
更新日期/Last Update: 2014-03-23