[1]方柏山,郑媛媛.酶体外定向进化(Ⅰ) 突变基因文库构建技术及其新进展[J].华侨大学学报(自然科学版),2004,25(4):337-342.[doi:10.3969/j.issn.1000-5013.2004.04.001]
 Fang Baishan,Zheng Yuanyuan.Directional Evolution of Enzymes in Vitro (Ⅰ) Technique for Generating Mutator Libraries and Its New Progress[J].Journal of Huaqiao University(Natural Science),2004,25(4):337-342.[doi:10.3969/j.issn.1000-5013.2004.04.001]
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酶体外定向进化(Ⅰ) 突变基因文库构建技术及其新进展()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第25卷
期数:
2004年第4期
页码:
337-342
栏目:
出版日期:
2004-10-20

文章信息/Info

Title:
Directional Evolution of Enzymes in Vitro (Ⅰ) Technique for Generating Mutator Libraries and Its New Progress
文章编号:
1000-5013(2004)04-0337-06
作者:
方柏山郑媛媛
华侨大学材料科学与工程学院; 华侨大学材料科学与工程学院 福建泉州362021; 福建泉州362021
Author(s):
Fang Baishan Zheng Yuanyuan
College of Mater. Sci. & Eng., Huaqiao Univ., 362021, Quanzhou, China
关键词:
定向进化 基因文库
Keywords:
enzyme directional evolution gene library
分类号:
Q785
DOI:
10.3969/j.issn.1000-5013.2004.04.001
文献标志码:
A
摘要:
介绍在酶体外定向进化研究中,几种常用基因文库构建技术及其应用方面的最新进展 .同时介绍近年来,一些新开发的基因文库的构建技术及其应用情况 .对目前几种主要方法的特色和缺陷进行讨论
Abstract:
Among the studies of directional evolution of enzymes in vitro, the authors provide information about the techniques in common use for generating gene libraries and the recent progress of their applications; and provide also information about techniques of new development for generating gene libraries and situation of their application. The characteristics and the limitation of several principel methods used at present are discussed.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目(20276026); 福建省自然科学重点基金资助项目(D01012); 福建省科技计划重点基金资助项目(2003I020)
更新日期/Last Update: 2014-03-23