[1]张光亚,方柏山.敏捷气热菌密码子及AUG侧翼序列保守性分析[J].华侨大学学报(自然科学版),2004,25(2):192-196.[doi:10.3969/j.issn.1000-5013.2004.02.021]
　Zhang Guangya,Fang Baishan.Analysing the Coden and the Conservative Character of AUG Context in Aeropyrum pernix[J].Journal of Huaqiao University(Natural Science),2004,25(2):192-196.[doi:10.3969/j.issn.1000-5013.2004.02.021]
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敏捷气热菌密码子及AUG侧翼序列保守性分析()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第25卷
期数:
2004年第2期
页码:
192-196
栏目:
出版日期:
2004-04-20

文章信息/Info

Title:
Analysing the Coden and the Conservative Character of AUG Context in Aeropyrum pernix
文章编号:
1000-5013(2004)02-0192-05
作者:
张光亚; 方柏山
华侨大学材料科学与工程学院; 华侨大学材料科学与工程学院 福建泉州362011; 福建泉州362011
Author(s):
Zhang Guangya;  Fang Baishan
College of Mater. Sci. & Eng., Huaqiao Univ., 362011, Quanzhou, China
关键词:
敏捷气热菌;  密码子使用偏好;  AUG侧翼序列;  碱基概率分布;  保守性
Keywords:
Aeropyrux pernix;  codon usage bias;  AUG context;  probability of base distribution;  conservative character
分类号:
Q933; Q811.4
DOI:
10.3969/j.issn.1000-5013.2004.02.021
文献标志码:
A
摘要:
比较敏捷气热菌和大肠杆菌等 9种编码区GC含量,以及各异生物的密码子使用情况 .结果表明,与敏捷气热菌编码区GC含量比较接近的果蝇,在密码子使用上与之相差最小 .它们在分类学上分别属于不同的域,与敏捷气热菌同属古菌,但GC含量相差较大的强烈炽热球菌,则相差较大 .说明编码区GC含量对密码子使用偏好,比生物分类学 (系统发育 )地位更重要 .碱基A,C在高、低表达基因中出现的概率差别较大,尤其在 - 1,- 3,- 4和 - 7位点; 碱基A,C在调控翻译起始效率中的作用,可能大于碱基G,U .因为碱基G,U在高表达和低表达基因中,其出现的概率差别不大 .高表达和低表达基因起始密码子侧翼序列中,某些位点保守性存在差异,其中高表达基因 - 4位和 - 3位可能与其高表达特性有关 .
Abstract:
Among 10 organisms with different coding GC contents, Aeropyrum pernix was compared with E.coli and others in their coden usage bias. least difference in coden usage bias was shown between A. pernix and Drosophila melanogaster, these two organism also were close in coding GC contents but they belong to two different domains in taxonomy. This illustrates that coding GC is more important than plilogenetic lineage for their coden usage bias. The probability of the occurrence of bases A and C in highly expressed genes and lowly expressed genes differred fairly greatly, esp in -1, -3, -4 and -7 sites. Bases A and C might be more important than bases G and U in the control of initial efficiency of translation. The conservative character of AUG context in highly expressed genes was shown to be different from that of lowly expressed genes. Of all the context sites, the highly expressed genes -4 and -3 were probably associated with their highly expressed character.

参考文献/References:

[1] Pamela Chavez, Croocker Yoshihiko, Sako Aritsune Uchida. Purification and characterization of an intracellular heat-stable proteinase (pernilase) from the marine hyperthermophilic archaeon Aeropyrum pernix K1 [J]. Extremophiles:Life Under Extreme Conditions, 1999(3):3-9.
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更新日期/Last Update: 2014-03-23