[1]万宁,李伟成,方柏山,等.细胞粗提液中木糖还原酶的催化特性考察[J].华侨大学学报(自然科学版),2001,22(2):194-198.[doi:10.3969/j.issn.1000-5013.2001.02.019]
　Wan Ning,Li Weicheng,Fang Baishan,et al.Property and Influencing Factors of Catalysis Induced by Xylose Reductase in Crude Extract of Candida Mogii Cells[J].Journal of Huaqiao University(Natural Science),2001,22(2):194-198.[doi:10.3969/j.issn.1000-5013.2001.02.019]
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细胞粗提液中木糖还原酶的催化特性考察()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第22卷
期数:
2001年第2期
页码:
194-198
栏目:
出版日期:
2001-04-20

文章信息/Info

Title:
Property and Influencing Factors of Catalysis Induced by Xylose Reductase in Crude Extract of Candida Mogii Cells
文章编号:
1000-5013(2001)02-0194-05
作者:
万宁; 李伟成; 方柏山; 苏志国
华侨大学材料科学与工程学院, 泉州362011; 中国科学院化工冶金研究所, 北京100080
Author(s):
Wan Ning1;  Li Weicheng2;  Fang Baishan1;  Su Zhiguo2
1.College of Mater. Sci. & Eng., Huaqiao Univ., 362011, Quanzhou; 2.Inst. of Chem. Metallurgy, Chinese Academy of Sciences, 100080, Beijing
关键词:
木糖还原酶;  辅酶;  细胞提取液;  木糖醇
Keywords:
xylose reductase;  coenzyme;  cell free extracts;  xylitol
分类号:
TQ03
DOI:
10.3969/j.issn.1000-5013.2001.02.019
摘要:
研究从 Candida Mogii细胞中,提取木糖还原酶 ( XR)的实验条件及 XR的催化动力学 .( 1)当破碎停留时间为 3min、离心转速为 12 0 0 0 r· min-1时,提取液中 XR的酶活保留较好 .( 2 )适当的 K+能够提高酶活,而 Mg2 +对 XR有抑制作用 .( 3)所得粗提液中的 XR具备一定的催化能力,可满足实际应用的要求 .( 4 )所得粗提液中的 XR半衰期约为 50 h,其稳定性还不足以实现长期连续催化生产 .
Abstract:
Candida Mogii cells are taken as object, from which xylose reductase(XR) is extracted. A study is made on experimental condition of extraction and catalytic kinetics of XR. As indicated by the study: (1) XR in crude extract keeps a fairly good enzyme activity under a crush retaining for 3 min. and a centrifugal speed up to 1 200 r·min -1; (2) enzyme activity of XR can be enhanced by K + in suitable concentration, but it will be inhibited by Mg 2+; (3) XR in crude extract has fixed catalytic force for satisfying the demand of practical use; and (4) XR in crude extract shows a half life about 50 hrs, its stability is inadequate for realizing long time continuous catalytic production.

相似文献/References:

[1]王晓霞,方柏山,李雯君.木糖还原酶基因在大肠杆菌中活性表达[J].华侨大学学报(自然科学版),2008,29(3):383.[doi:10.11830/ISSN.1000-5013.2008.03.0383]
　WANG Xiao-xia,FANG Bai-shan,LI Wen-jun.Expression of Xylose Reductase Genes from Candida shehatae in E.coli[J].Journal of Huaqiao University(Natural Science),2008,29(2):383.[doi:10.11830/ISSN.1000-5013.2008.03.0383]
[2]杨仲丽,方柏山,余劲聪,等.辅酶依赖型和不依赖型甘油脱水酶的比较[J].华侨大学学报(自然科学版),2010,31(5):542.[doi:10.11830/ISSN.1000-5013.2010.05.0542]
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备注/Memo

备注/Memo:
国家自然科学基金
更新日期/Last Update: 2014-03-23