[1]胡纯铿,王国川.混合培养发酵L-苹果酸的研究[J].华侨大学学报(自然科学版),2000,21(1):76-79.[doi:10.3969/j.issn.1000-5013.2000.01.016]
　Hu Chunkeng,Wang Guochuan.A Study on L-Malic Acid Fermentation by Mixed Culture[J].Journal of Huaqiao University(Natural Science),2000,21(1):76-79.[doi:10.3969/j.issn.1000-5013.2000.01.016]
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混合培养发酵L-苹果酸的研究()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第21卷
期数:
2000年第1期
页码:
76-79
栏目:
出版日期:
2000-01-20

文章信息/Info

Title:
A Study on L-Malic Acid Fermentation by Mixed Culture
文章编号:
1000-5013(2000)01-0076-04
作者:
胡纯铿; 王国川
华侨大学化工学院, 泉州362011; 福建惠泉啤酒集团股份有限公司, 惠安362100
Author(s):
Hu Chunkeng1;  Wang Guochuan2
1.College of Chem. Eng., Huaqiao Univ., 362011, Quanzhou; 2.Fujian Huiquan Brewage Group Inc., Huian, 362100
关键词:
L-苹果酸;  混合发酵;  无根根霉;  普通变形杆菌
Keywords:
L malic acid;  mixed fermentation;  Rhizopus arrhizus;  Proteus Vulgaris
分类号:
TQ921
DOI:
10.3969/j.issn.1000-5013.2000.01.016
摘要:
采用 A- 2 3菌株和 V- 81菌株混合培养发酵 L -苹果酸,分别探讨碳源浓度对 A- 2 3菌株和 V-81菌株发酵水平的影响 .试验表明,A- 2 3菌株产延胡索酸能力强,V- 81菌株具有高活性延胡索酸酶 .在此基础上,研究 A- 2 3菌株和 V- 81菌株混合培养方式对发酵 L-苹果酸的影响 .结果表明,A- 2 3菌株接入含有 150 g· L-1葡萄糖的混合发酵培养基中培养 3d,后接入 V- 81菌株并继续培养 2 d,产酸水平最高,L-苹果酸浓度达 73.3g· L-1,对投糖浓度的摩尔转化率达 6 5.6 % .
Abstract:
Lmalic acid fermentation was performed by a mixed culture of strain A 23 and strain V 81. The effect of concentration of carbon source on the fermentation level of both strain A 23 and strain V 81 was investigated. The experimental results showed that strain A 23 was very capable of producing fumaric acid while strain V 81 had strong fumarase activity. On the basis of above investigation, the influence of the way for a mixed culture of strain A 23 and strain V 81 on L malic acid fermantation was further studied. The result indicated that the highest L malic acid yield was acquired when strain A 23 was inoculated and cultured in the mixed fermentation medium containing 150 g·L -1 glucose for 3 days, then strain V 81 was inoculated and cultured for 2 days. The concentration of L malic acid reached 73.3 g·L -1 while its molar conversion rate to initial glucose concentration in the medium amounted to 65.6%.

参考文献/References:

[1] 胡纯铿. L-苹果酸产生菌黄曲霉Aspergillus flavus H-98发酵特性的研究 [J]. 食品与发酵工业, 1999(2):19-22.doi:10.3321/j.issn:0253-990X.1999.02.005.
[2] 胡纯铿. 固定化黄曲霉生产L-苹果酸的研究 [J]. 华侨大学学报(自然科学版), 1999(3):240-245.
[3] 金其荣. L-苹果酸产生菌的选育 [J]. 无锡轻工学院学报, 1982.89-97.
[4] Takao S, Hotta K. Conversion of fumaric acid fermentation to L-malic acid fermentation by the association of Rhizopus arrhizus and Proteus vulgaris [J]. Journal of Fermentation and Technology, 1976(4):197-204.
[5] Takao S, Yokota A, Tanida M. L-Malic acid fermentation by a mixed culture of Rhizopus arrhizus and Paecilomyces varioti [J]. Journal of Fermentation and Technology, 1983(6):643-645.
[6] 蒋明珠, 白照熙, 张俊贤. 无根根霉R25和普通变形杆菌P1混合培养发酵L-苹果酸的研究 [J]. 微生物学报, 1989(2):129-136.
[7] Yamamoto K, Tosa T, Yamashita K. The production of L-malic acid by Brevibacterium ammoniagenes immobilized with polyacrylamide gel [J]. European Journal of Applied Microbiology, 1976(3):169-175.doi:10.1007/BF01385432.
[8] Takata I, Kayashima K, Tosa T. Improvement of stability of fumarase activity of Brevibacterium flavum by immobilization with κ-carrageean and polyethyleneimine [J]. Journal of Fermentation and Technology, 1982(5):431-437.
[9] Yakawa H, Yamagata H, Terasawa M. Production of L-malic acid by the cell reusing process [J]. Process Biochemistry, 1986.164-166.
[10] 胡纯铿. 细胞转化法生产L-苹果酸的研究 [J]. 华侨大学学报(自然科学版), 1999(4):349-353.
[11] Goodban A E, Stark J B. A method for rapid determination of L-malic acid [J]. Analytical Chemistry, 1957.283-287.doi:10.1021/ac60122a032.
[12] 北京大学生物系生物化学教研室. 生物化学实验指导 [M]. 北京:高等教育出版社, 1985.30-31.

备注/Memo

备注/Memo:
福建企业横向科研合作基金资助项目
更新日期/Last Update: 2014-03-23