[1]林莺.洁霉素产生菌原生质体的形成、再生和融合[J].华侨大学学报(自然科学版),1989,10(3):331-336.[doi:10.11830/ISSN.1000-5013.1989.03.0331]
Lin Ying.The Formation, Regeneration and Recombination of Protoplasts from Lincomycinum Productive Strain[J].Journal of Huaqiao University(Natural Science),1989,10(3):331-336.[doi:10.11830/ISSN.1000-5013.1989.03.0331]
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洁霉素产生菌原生质体的形成、再生和融合(
)
《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]
- 卷:
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第10卷
- 期数:
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1989年第3期
- 页码:
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331-336
- 栏目:
-
- 出版日期:
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1989-07-20
文章信息/Info
- Title:
-
The Formation, Regeneration and Recombination of Protoplasts from Lincomycinum Productive Strain
- 作者:
-
林莺
-
华侨大学化工与生化工程系
- Author(s):
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Lin Ying
-
-
- 关键词:
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洁酶素; 原生质体; 溶菌酶
- Keywords:
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lincomycinum; protoplast; lysozyme
- DOI:
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10.11830/ISSN.1000-5013.1989.03.0331
- 摘要:
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洁霉素产生菌不论生长在含有甘氨酸的S培养基或不含甘氨酸的S培养基的菌丝中,其细胞壁均可被溶菌酶溶解而释放出原生质体。甘氨酸浓度为1.0%破壁效果最好,溶菌酶的作用浓度以2.5mg/ml为最佳。原生质体在Hb培养基上能够很好地再生。用P培养基配制的40%(w/v)PEG1000溶液,能有效地诱导洁霉素产生菌原生质体融合。融合重组频率达6.7%。
- Abstract:
-
This paper studies the formation, regeneration and recombination of proto- plasts from the mycelia of lincomycinum productive strain which grown on S medium containing glycine or not. The cell wall of mycelia can be solved by ly- sozyme and thus protoplas
更新日期/Last Update:
2014-03-22